IL1A Antikörper
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- Target Alle IL1A Antikörper anzeigen
- IL1A (Interleukin 1 alpha (IL1A))
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Reaktivität
- Ratte
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Wirt
- Ziege
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Klonalität
- Polyklonal
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Konjugat
- Dieser IL1A Antikörper ist unkonjugiert
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Applikation
- Western Blotting (WB), ELISA, Neutralization (Neut)
- Spezifität
- Rat IL-1alpha
- Aufreinigung
- Immunoaffinity purified
- Immunogen
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E.coli derived recombinant Rat IL-1alpha.
Type of Immunogen: Cell extract - Top Product
- Discover our top product IL1A Primärantikörper
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- Applikationshinweise
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Approved: ELISA (0.5 - 2 μg/mL), Neut (11 - 17 μg/mL), WB
Usage: Immunohistochemistry: This antibody stained colchicine injected rat brain (including the ventricles and the CA3 region of the hippocampus) tissue. The primary antibody was incubated at 1 μg/mL overnight at 4 °C. This was followed by a peroxidase conjugated secondary antibody and then a fluorescein Tyramide Signal Amplification (TSA) reagent. Optimal concentrations and conditions may vary. Information and photo are courtesy of the Tissue Profiling group, SciLifeLab Stockholm. Neutralization: To yield one-half maximal inhibition [ND] of the biological activity of Rat IL-1alpha (50 pg/mL), a concentration of 0.011-0.017 μg/mL of this antibody is required. ELISA: To detect Rat IL-1alpha by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5-2 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Rat IL-1alpha (LS-C104576) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant Rat IL-1alpha. Western Blot: To detect Rat IL-1alpha by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant Rat IL-1alpha is 1.5-3 ng/lane, under either reducing or non-reducing conditions. - Kommentare
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Target Species of Antibody: Rat
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Format
- Lyophilized
- Rekonstitution
- Sterile water
- Konzentration
- Lot specific
- Buffer
- Lyophilized from PBS, pH 7.2
- Handhabung
- Avoid freeze/thaw cycles.
- Lagerung
- 4 °C,-20 °C
- Informationen zur Lagerung
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Store Lyophilized at room temperature up to 1 month
Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.
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- Target
- IL1A (Interleukin 1 alpha (IL1A))
- Andere Bezeichnung
- IL1A / IL1 Alpha (IL1A Produkte)
- Synonyme
- IL-1A antikoerper, IL1 antikoerper, IL1-ALPHA antikoerper, IL1F1 antikoerper, IL-6 antikoerper, Il-1a antikoerper, IL-1 alpha antikoerper, IL-1alpha antikoerper, L1A antikoerper, interleukin 1 alpha antikoerper, interleukin 6 antikoerper, IL1A antikoerper, IL6 antikoerper, Il1a antikoerper
- Hintergrund
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Name/Gene ID: IL1A
Family: IL-1
Synonyms: IL1A, Hematopoietin-1, IL-1 alpha, IL1, IL1-ALPHA, IL1 Alpha, Interleukin-1 alpha, IL-1A, IL1F1, Pro-interleukin-1-alpha, Preinterleukin 1 alpha, Interleukin 1, alpha - Gen-ID
- 3552
- UniProt
- P01583
- Pathways
- NF-kappaB Signalweg, Autophagie, Cancer Immune Checkpoints
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