NOV Antikörper

Produktnummer ABIN465355

Produktdetails anti-NOV Antikörper

Target: NOV (Nephroblastoma Overexpressed (NOV))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser NOV Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA

Spezifität: Human NOV

Aufreinigung: Immunoaffinity purified

Immunogen: E.coli derived recombinant Human NOV.

Type of Immunogen: Cell extract

Anwendungsinformationen

Applikationshinweise: Approved: ELISA, WB (0.1 - 0.2 μg/mL)

Usage: Immunohistochemistry: This antibody stained formalin-fixed, paraffin-embedded sections of human breast invasive ductal carcinoma. The recommended concentration is 0.25 μg/mL-0.50 μg/mL with an overnight incubation at 4 °C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a  pH 6 sodium citrate buffer is recommended. Optimal concentrations and conditions may vary. ELISA: To detect hNOV by sandwich ELISA (using 100 μ.l/well antibody solution) a concentration of 0.5-2 μ.g/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human NOV (LS-C104549) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hNOV. Western Blot: To detect hNOV by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hNOV is 1.5-3 ng/lane, under either reducing or non-reducing conditions.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Sterile water

Konzentration: Lot specific

Buffer: Lyophilized from PBS,  pH 7.2

Handhabung: Avoid freeze/thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store Lyophilized at room temperature up to 1 month
Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.

Details zu NOV

Target: NOV (Nephroblastoma Overexpressed (NOV))

Andere Bezeichnung: CCN3 / NOV (NOV Produkte)

Synonyme: CCN3 antikoerper, IBP-9 antikoerper, IGFBP-9 antikoerper, IGFBP9 antikoerper, NOVh antikoerper, nov-A antikoerper, xnov antikoerper, NovH antikoerper, C130088N23Rik antikoerper, nephroblastoma overexpressed antikoerper, nephroblastoma overexpressed L homeolog antikoerper, nephroblastoma overexpressed gene antikoerper, NOV antikoerper, nov.L antikoerper, Nov antikoerper

Hintergrund: Name/Gene ID: NOV

Synonyms: NOV, CCN3, CCN family member 3, IGFBP9, IBP-9, IGFBP-9, NOVh, IGF-binding protein 9, Nephroblastoma overexpressed, Protein NOV homolog

Gen-ID: 4856

UniProt: P48745

Pathways: Smooth Muscle Cell Migration, Growth Factor Binding