TYROBP Antikörper

Produktnummer ABIN462404

Produktdetails anti-TYROBP Antikörper

Target: TYROBP (TYRO Protein tyrosine Kinase Binding Protein (TYROBP))

Reaktivität: Human, Fledermaus, Hund, Pferd, Affe

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser TYROBP Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (IHC), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Marke: IHC-plus™

Spezifität: Human TYROBP / DAP12. This antibody is expected to recognize both reported isoforms (NP_003323.1, NP_937758.1)

Homologie: Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Marmoset, Dog, Bat, Horse (100%) Sheep, Panda, Rabbit (92%) Mouse, Elephant, Zebu, Bovine (85%).

Aufreinigung: Immunoaffinity purified

Immunogen: Synthetic peptide C-KQRITETESPYQE from an internal region of human TYROBP / DAP12 (NP_003323.1, NP_937758.1). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Marmoset, Dog, Bat, Horse (100%), Sheep, Panda, Rabbit (92%), Mouse, Elephant, Zebu, Bovine (85%).

Type of Immunogen: Synthetic peptide

Anwendungsinformationen

Applikationshinweise: Approved: IHC, IHC-P (3 - 6 μg/mL), Peptide-ELISA

Usage: Peptide ELISA: antibody detection limit dilution 1:32000. Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 3-6 μg/mL.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Store at -20°C. Avoid freeze-thaw cycles.

Details zu TYROBP

Target: TYROBP (TYRO Protein tyrosine Kinase Binding Protein (TYROBP))

Andere Bezeichnung: DAP12 (TYROBP Produkte)

Hintergrund: Name/Gene ID: TYROBP

Synonyms: TYROBP, DNAX-activation protein 12, DAP12, PLOSL, KARAP, KAR-associated protein, PLO-SL

Gen-ID: 7305

NCBI Accession: NP_003323, NP_937758

UniProt: O43914