Septin 2 Antikörper (AA 37-49)

Produktnummer ABIN462293

Produktdetails anti-Septin 2 Antikörper

Target: Septin 2 (SEPT2)

Bindungsspezifität: AA 37-49

Reaktivität: Human, Maus, Ratte, Kaninchen

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser Septin 2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Marke: IHC-plus™

Spezifität: Human SEPT2 / Septin 2. Reported variants represent identical protein: NP_001008492.1, NP_006146.1, NP_004395.1, NP_001008491.1.

Homologie: Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Mouse, Rat, Rabbit (100%) Marmoset, Bovine, Dog, Horse (92%) Panda, Bat, Pig, Chicken, Platypus (85%).

Aufreinigung: Immunoaffinity purified

Immunogen: Synthetic peptide SKQQPTQFINPET-C from the N-terminus of human SEPT2 / Septin 2 (NP_001008491.1). Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Mouse, Rat, Rabbit (100%), Marmoset, Bovine, Dog, Horse (92%), Panda, Bat, Pig, Chicken, Platypus (85%).

Type of Immunogen: Synthetic peptide

Anwendungsinformationen

Applikationshinweise: Approved: IHC, IHC-P (2.5 μg/mL), Peptide-ELISA (1:32000, 0.05 - 0.2 μg/mL), WB (0.05 - 0.2 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 2.5 μg/mL. Peptide ELISA: antibody detection limit dilution 1:32000. Western Blot: Approx. 40kD band observed in Human, Mouse and Rat Testis lysates (calculated MW of 41.5kD according to NP_001008491.1). Recommended concentration: 0.05-0.2 μg/mL.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Store at -20°C. Avoid freeze-thaw cycles.

Details zu Septin 2

Target: Septin 2 (SEPT2)

Andere Bezeichnung: SEPT2 / Septin 2 (SEPT2 Produkte)

Synonyme: DIFF6 antikoerper, NEDD5 antikoerper, Pnutl3 antikoerper, hNedd5 antikoerper, AW208991 antikoerper, Nedd-5 antikoerper, Nedd5 antikoerper, mKIAA0158 antikoerper, Vesp11 antikoerper, nedd5 antikoerper, wu:fb52g01 antikoerper, wu:fb99a01 antikoerper, Septin-2A antikoerper, Septin-A antikoerper, diff6 antikoerper, pnutl3 antikoerper, sept2 antikoerper, septa antikoerper, xlsepta antikoerper, SEPT2 antikoerper, Sep-02 antikoerper, Septin-2B antikoerper, hnedd5 antikoerper, sept2-B antikoerper, Septin-2 antikoerper, septin 2 antikoerper, septin 2 L homeolog antikoerper, septin 2-like antikoerper, COG1487; VapC; putative nucleic acid-binding protein; contains PIN domain antikoerper, septin 2 S homeolog antikoerper, SEPT2 antikoerper, Sept2 antikoerper, sept2 antikoerper, sept2.L antikoerper, SEPT2L antikoerper, sepT2 antikoerper, sept2.S antikoerper

Hintergrund: Name/Gene ID: SEPT2

Synonyms: SEPT2, DIFF6, HNedd5, KIAA0158, NEDD5, Septin-2, Septin 2, NEDD-5, PNUTL3

Gen-ID: 4735

NCBI Accession: NP_001008491

UniProt: Q15019