GRIA4 Antikörper (AA 279-292)

Produktnummer ABIN462292

Produktdetails anti-GRIA4 Antikörper

Target: GRIA4 (Glutamate Receptor, Ionotropic, AMPA 4 (GRIA4))

Bindungsspezifität: AA 279-292

Reaktivität: Human, Maus, Ratte, Huhn, Affe, Rind (Kuh), Hund, Pferd, Kaninchen, Fledermaus

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser GRIA4 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Marke: IHC-plus™

Spezifität: Human GRIA4 / GLUR4. This antibody is expected to recognize all reported isforms (NP_000820.3, NP_001070711.1, NP_001070712.1). Reported variants NP_001070712.1 and NP_001106283.1 represent identical protein.

Homologie: Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Elephant, Panda, Dog, Bat, Bovine, Horse, Rabbit, Opossum, Turkey, Chicken, Platypus (100%) Lizard (93%) Xenopus, Stickleback, Pufferfish, Zebrafish, Trachemys (86%).

Aufreinigung: Immunoaffinity purified

Immunogen: Synthetic peptide C-KKLDQREYPGSETP from an internal region of human GRIA4 / GLUR4 (NP_000820.3, NP_001070711.1, NP_001070712.1). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Elephant, Panda, Dog, Bat, Bovine, Horse, Rabbit, Opossum, Turkey, Chicken, Platypus (100%), Lizard (93%), Xenopus, Stickleback, Pufferfish, Zebrafish, Trachemys (86%).

Type of Immunogen: Synthetic peptide

Anwendungsinformationen

Applikationshinweise: Approved: IHC, IHC-P (5 μg/mL), Peptide-ELISA (1:128000, 0.3 - 1 μg/mL), WB (0.3 - 1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Peptide ELISA: antibody detection limit dilution 1:128000. Western Blot: Approx. 90 kD band observed in Human Brain (Cerebellum) lysates (calculated MW of 99.2 kD according to NP_001070711.1). Recommended concentration: 0.3-1 μg/mL.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Store at -20°C. Avoid freeze-thaw cycles.

Details zu GRIA4

Target: GRIA4 (Glutamate Receptor, Ionotropic, AMPA 4 (GRIA4))

Andere Bezeichnung: GRIA4 / GLUR4 (GRIA4 Produkte)

Synonyme: GLUR4 antikoerper, GLUR4C antikoerper, GLURD antikoerper, GluA4 antikoerper, GluR4 antikoerper, GluR4d antikoerper, GLUR4/D antikoerper, glur4 antikoerper, GluR-D antikoerper, Glur-4 antikoerper, Glur4 antikoerper, Gluralpha4 antikoerper, spkw1 antikoerper, DKFZp459P0616 antikoerper, glur4a antikoerper, gria4 antikoerper, iGluR4 antikoerper, ZfGluR4b antikoerper, fj46g06 antikoerper, glur4b antikoerper, wu:fj46g06 antikoerper, glutamate ionotropic receptor AMPA type subunit 4 antikoerper, glutamate receptor, ionotropic, AMPA 4 antikoerper, glutamate receptor, ionotropic, AMPA4 (alpha 4) antikoerper, glutamate receptor, ionotropic, AMPA 4a antikoerper, glutamate receptor, ionotropic, AMPA 4b antikoerper, GRIA4 antikoerper, gria4 antikoerper, Gria4 antikoerper, gria4a antikoerper, gria4b antikoerper

Hintergrund: Name/Gene ID: GRIA4
Subfamily: Glutamate receptor - ionotropic (NMDA receptor)
Family: Ion Channel

Synonyms: GRIA4, GluA4, GluR-4, GLUR4, GLUR4C, Glutamate receptor type d, GluR-D, GLURD, Glutamate receptor 4

Gen-ID: 2893

NCBI Accession: NP_000820, NP_001070711, NP_001070712

UniProt: P48058

Pathways: PI3K-Akt Signalweg