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ASH2L Antikörper (AA 288-337)

ASH2L Reaktivität: Human, Maus, Ratte, Hund, Pferd, Rind (Kuh), Meerschweinchen, Affe, Fledermaus, Kaninchen, Schwein, Hamster WB, IHC, IHC (p) Wirt: Kaninchen Polyclonal unconjugated
Produktnummer ABIN6741556
  • Target Alle ASH2L Antikörper anzeigen
    ASH2L (Set1/Ash2 Histone Methyltransferase Complex Subunit ASH2 (ASH2L))
    Bindungsspezifität
    • 6
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 288-337
    Reaktivität
    • 50
    • 27
    • 23
    • 10
    • 8
    • 8
    • 6
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Human, Maus, Ratte, Hund, Pferd, Rind (Kuh), Meerschweinchen, Affe, Fledermaus, Kaninchen, Schwein, Hamster
    Wirt
    • 45
    • 7
    • 1
    Kaninchen
    Klonalität
    • 34
    • 19
    Polyklonal
    Konjugat
    • 36
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    Dieser ASH2L Antikörper ist unkonjugiert
    Applikation
    • 33
    • 17
    • 17
    • 11
    • 7
    • 6
    • 6
    • 5
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
    Marke
    IHC-plus™
    Spezifität
    Human ASH2L
    Homologie
    Percent identity by BLAST analysis: Human, Mouse, Rat, Dog, Horse, Guinea pig (100%) Bovine, Chicken (85%).
    Aufreinigung
    Immunoaffinity purified
    Immunogen
    Synthetic peptide located between aa288-337 of human ASH2L (Q9UBL3, NP_004665). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Galago, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Dog, Bat, Rabbit, Horse, Pig, Opossum, Guinea pig, Platypus (100%), Bovine, Turkey, Chicken (85%).

    Type of Immunogen: Synthetic peptide
    Isotyp
    IgG
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  • Applikationshinweise
    Approved: IHC, IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)

    Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody. ELISA titer in peptide based assay: 1:1562500.
    Kommentare

    Target Species of Antibody: Human

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Lyophilized
    Rekonstitution
    Distilled water
    Konzentration
    Lot specific
    Buffer
    Lyophilized from PBS with 2 % sucrose
    Handhabung
    Avoid repeat freeze-thaw cycles.
    Lagerung
    4 °C,-20 °C
    Informationen zur Lagerung
    Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
    Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
  • Target
    ASH2L (Set1/Ash2 Histone Methyltransferase Complex Subunit ASH2 (ASH2L))
    Andere Bezeichnung
    ASH2L / ASH2 (ASH2L Produkte)
    Synonyme
    ASH2 antikoerper, ASH2L1 antikoerper, ASH2L2 antikoerper, Bre2 antikoerper, fb34c06 antikoerper, fe38b04 antikoerper, fi20h02 antikoerper, wu:fb34c06 antikoerper, wu:fe38b04 antikoerper, wu:fi20h02 antikoerper, zgc:171444 antikoerper, ASH2L antikoerper, ash2-like antikoerper, ASH2 like histone lysine methyltransferase complex subunit antikoerper, ash2 (absent, small, or homeotic)-like (Drosophila) antikoerper, ash2 (absent, small, or homeotic)-like L homeolog antikoerper, ASH2L antikoerper, Ash2l antikoerper, ash2l antikoerper, ash2l.L antikoerper
    Hintergrund
    Name/Gene ID: ASH2L

    Synonyms: ASH2L, ASH2, ASH2-like protein, ASH2L1, ASH2L2, Bre2
    Gen-ID
    9070
    NCBI Accession
    NP_004665
    UniProt
    Q9UBL3
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