The reactivity of the antiserum is directed to the major isotypes of the cat immunoglobulin system as tested by immunoelectrophoresis and double radial immunodiffusion against pooled serum and purified immunoglobulinsPrecipitating polyclonal goat antiserum to cat immunoglobulins, heavy and light chains
Produktmerkmale
Total protein and IgG concentration in the antiserum are comparable to those of pooled goat serum. Antibody titre: Precipitin titre not less than 1:64 when tested against normal cat serum in agar block titration.
Aufreinigung
Delipidated, heat inactivated, lyophilized stable whole serum Immunoaffinity adsorbed using insolubilized antigens as required to eliminate antibody activity with other serum protein. The use of insolubilized adsorption antigens prevents the presence of excess adsorbent protein or immune complexes in the antiserum.
Immunogen
Purified normal IgG and fractions containing IgA and IgM and isolated from cat serum. Freund's complete adjuvant is used in the first step of the immunization procedure.
Isotyp
IgG
Applikationshinweise
In precipitating techniques as immunoelectrophoresis and single and double radial immunodiffusion (Mancini, Ouchterlony) to identify and measure immunoglobulins, as secondary antibody to precipitate the immunoglobulin in normal cat serum.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Lyophilized
Rekonstitution
It is reconstituted by adding 1 mL sterile distilled water.
Buffer
No foreign proteins added.
Konservierungsmittel
Without preservative
Handhabung
Working dilutions are prepared by adding sterile phosphate buffered saline (PBS, pH 7. 2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4 °C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product.
Lagerung
4 °C/-20 °C
Informationen zur Lagerung
The lyophilized antiserum is shipped at ambient temperature and may be stored at +4 °C, prolonged storage at or below -24 °C.