Human Papilloma Virus 18 E2 (HPV-18 E2) (AA 1-83) Antikörper

Produktnummer ABIN457548

Produktdetails

Target: Human Papilloma Virus 18 E2 (HPV-18 E2)

Bindungsspezifität: AA 1-83

Reaktivität: Human Papillomavirus (HPV)

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Unkonjugiert

Applikation: ELISA, Western Blotting (WB), Immunofluorescence (IF)

Spezifität: Reacts with HPV-18 E2-protein.

Aufreinigung: purified with Protein G affinity chromatorgaphy

Immunogen: Recombinant full-length HPV-18 E2-protein purifiedfrom E. coli

Klon: 2E7

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: ELISA:1: 2 000, WB: 1: 1000. This antibody does not work in IP. Monoclonal antibody working titer has to be established practically for each particular antigen and assay format

Beschränkungen: Nur für Forschungszwecke einsetzbar

Independent Validation (UP)

Validierung #102723 (Unfolding Profile)

by: NanoTemper Technologies

No.: #102723

Datum: 05.11.2018

Antigen: HPV-18

Chargennummer: A10P2E71-001

Validierte Anwendung: Unfolding Profile

Positivkontrolle: ABIN457548

Bewertung:

Passed. ABIN457548 showed a clear unfolding profile with two detected unfolding events with T_is at 78.4°C and 84.4°C. It is not unusual for antibodies to show more than one unfolding transition, as they are composed of distinct domains that may unfold independently and at different temperatures. This result suggests that the protein is properly folded and functional.

Validierungsbilder

Unfolding profile of ABIN457548. The fluorescence signal is plotted against temperature. The native (folded) protein has a low signal at the beginning of the experiment, which increases upon unfolding, showing two unfolding events. The vertical lines indicate the Tis at 78.4 °C and 84.4 °C.

Protokoll

Primärantikörper: ABIN457548

Full Protocol:

• Dilute ABIN457548 1:10 in PBS buffer (Roth, 1058.1, lot 285231988) to get a final volume of 15µl at a concentration of 0.67µM.
• Load sample into Tycho capillary (NanoTemper Technologies, TY-C001).
• Run Tycho measurement.

Anmerkungen:

Tycho is designed to run quick and precise protein quality check experiments. Tycho uses intrinsic protein fluorescence to follow protein unfolding while running a fast thermal ramp, yielding results in 3min. A protein’s unfolding behavior is characterized by various parameters, most notably the inflection temperature (T_i). The T_i can be used to identify properly folded protein, to compare different batches, or to analyze the influence of storage/transport conditions on a protein. An absence of T_i would suggest that the protein is already unfolded and therefore most likely nonfunctional.

Handhabung

Format: Liquid

Konzentration: 1 mg/ml

Buffer: PBS pH 7.4, with 0.1%

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Divide antibody into aliquots prior usage. Avoid multiple freeze-thaw cycles.

Lagerung: -20 °C

Informationen zur Lagerung: This product is shipped in non-frozen liquid form in ambient conditions

Antigendetails

Target: Human Papilloma Virus 18 E2 (HPV-18 E2)

Substanzklasse: Viral Protein

Hintergrund: Human papillomaviruses(HPV) are small DNA viruses whichinfect epithelia of the skin and mucosa. Over 90 types have been identified andthey mostly cause a variety of benign lesions such as warts and verrucae.However, some subtypes, notably types 16 and 18, 31 and 33, have been confirmedas agents which cause cervical cancer. Human Papillomavirus (HPV) E7 proteins havecellular transforming and trans-activating activities