ELISA, Western Blotting (WB), Flow Cytometry (FACS), Functional Studies (Func)
Spezifität
Human IgE, Fce3-4, no cross-ractivity with other human or murine Ab isotypes
Sensitivität
Reactivity > 95 %, compared to calibrator (Internal Standard mAb clone BSW17), tested by ELISA binding to immobilized human Standard IgE (clone: SUS-11)
Produktmerkmale
IGHE, chromosome 14q32.33
Aufreinigung
Affinity - purified from cell culture supernatant by Immunoaffinity Chromatography on CH-Sepharose 4B - rat monoclonal anti- mouse Ck
Reinheit
> 85 %, tested by analytical Gel Permeation Chromatography
The antibody recognizes a conformational epitope within the Ce3 domain of human IgE spanning towards the amino-terminal part of the Ce4 domain. It blocks the binding of IgE to its high affinity receptor on mast cells and basophils. It is therefore suited as an inhibitory reference compound for mast cell modulator profiling and basic research on IgE mediated mast cell receptor signaling.