PRR7 Antikörper (C-Term, Intracellular)

Produktnummer ABIN457438

Produktdetails anti-PRR7 Antikörper

Target: PRR7 (Proline Rich 7 (Synaptic) (PRR7))

Bindungsspezifität: C-Term, Intracellular

Reaktivität: Human, Maus, Ratte

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser PRR7 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunocytochemistry (ICC)

Spezifität: The mouse monoclonal antibody TRAP3/10 recognizes an epitope located in the C-terminal part of the intracellular domain of PRR7/TRAP3 (amino acids 126-253 of human PRR7 / TRAP3), a 28 kDa proline-rich membrane protein presumably associated with NMDA receptor complex.

Kreuzreaktivität (Details): Human, Mouse, Rat

Aufreinigung: Purified by protein-A affinity chromatography.

Reinheit: > 95 % (by SDS-PAGE)

Immunogen: Recombinant C-terminal half of the intracellular domain of human PRR7/TRAP3 (amino acids 126-253)

Klon: TRAP3-10

Isotyp: IgG2a

Anwendungsinformationen

Applikationshinweise: Immunocytochemistry: Recommended dilution: 10 μg/mL, cell culture fixed with 4 % paraformaldehyde, permeabilized with 0.1 % Triton-X100.
Western blotting: Recommended dilution: 1 μg/mL, positive control: murine brain lysate (red. Laemmli buffer).

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Do not freeze.

Lagerung: 4 °C

Informationen zur Lagerung: Store at 2-8°C. Do not freeze.

Referenzen für anti-PRR7 Antikörper (ABIN457438)

Hrdinka, Dráber, Stepánek, Ormsby, Otáhal, Angelisová, Brdicka, Paces, Horejsí, Drbal: "PRR7 is a transmembrane adaptor protein expressed in activated T cells involved in regulation of T cell receptor signaling and apoptosis." in: The Journal of biological chemistry, Vol. 286, Issue 22, pp. 19617-29, (2011) (PubMed).

Details zu PRR7

Target: PRR7 (Proline Rich 7 (Synaptic) (PRR7))

Andere Bezeichnung: PRR7 / TRAP3 (PRR7 Produkte)

Synonyme: PRR7 antikoerper, XM_484263 antikoerper, proline rich 7, synaptic antikoerper, proline rich 7 (synaptic) antikoerper, proline rich 7 (synaptic) L homeolog antikoerper, PRR7 antikoerper, prr7 antikoerper, prr7.L antikoerper, Prr7 antikoerper

Hintergrund: Proline rich 7, synaptic,PRR7/TRAP3 (proline-rich 7, transmembrane adaptor protein 3) is a 28 kDa transmembrane adaptor protein ubiquitously expressed at low level (most in brain). Its amino acid sequence is extremely conserved among mammalian and other species. PRR7/TRAP3 contains potential palmitoylation motif and is found in lipid rafts. It is a part of the complex postsynaptic density fraction in neurons and associates with PSD-95, NMDA receptor and probably other proteins. The intracellular domain of PRR7/TRAP3 contains several tyrosines, a proline-rich sequence, and a C-terminal PDZ-binding motif. So far nothing is known about function of this protein. It may be involved in regulation of some receptor signaling and in formation of neurologic and immunologic synapse.,TRAP3

Gen-ID: 80758

UniProt: Q8TB68