ITGAL Antikörper

Produktnummer ABIN457403

Produktdetails anti-ITGAL Antikörper

Target: ITGAL (Integrin alpha-L (ITGAL))

Reaktivität: Maus

Wirt: Ratte

Klonalität: Monoklonal

Konjugat: Dieser ITGAL Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS), Immunoprecipitation (IP), Functional Studies (Func), Immunohistochemistry (Frozen Sections) (IHC (fro))

Spezifität: The rat monoclonal antibody M17/4 reacts with an extracellular epitope of CD11a (alpha-subunit of murine LFA-1), a 180 kDa type I transmembrane glycoprotein expressed on B and T lymphocytes, monocytes, macrophages, neutrophils, basophils and eosinophils.

Kreuzreaktivität (Details): Mouse

Aufreinigung: Purified by protein-G affinity chromatography.

Reinheit: > 95 % (by SDS-PAGE)

Endotoxin-Niveau: Endotoxin level is less than 0.01 EU/µg of the protein, as determined by the LAL test.

Immunogen: C57BL/6 mouse splenic secondary cytotoxic T lymphocytes

Klon: M17-4

Isotyp: IgG2a

Anwendungsinformationen

Applikationshinweise: Functional application: Blocking.
Flow cytometry: Recommended dilution: 1 μg/mL.
Immunohistochemistry (frozen sections): Positive tissue: murine spleen or thymus, acetone fixation.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4

Konservierungsmittel: Azide free

Handhabung: Do not freeze.

Lagerung: 4 °C

Informationen zur Lagerung: Store at 2-8°C. Do not freeze.

Referenzen für anti-ITGAL Antikörper (ABIN457403)

Smith, Barnum: "Differential expression of beta 2-integrins and cytokine production between gammadelta and alphabeta T cells in experimental autoimmune encephalomyelitis." in: Journal of leukocyte biology, Vol. 83, Issue 1, pp. 71-9, (2007) (PubMed).

Angelov, Guillaume, Cebecauer, Bosshard, Dojcinovic, Baumgaertner, Luescher: "Soluble MHC-peptide complexes containing long rigid linkers abolish CTL-mediated cytotoxicity." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 176, Issue 6, pp. 3356-65, (2006) (PubMed).

Beyer, Wang, Peters, Doths, Koerner-Rettberg, Openshaw, Schwarze: "The beta2 integrin CD11c distinguishes a subset of cytotoxic pulmonary T cells with potent antiviral effects in vitro and in vivo." in: Respiratory research, Vol. 6, pp. 70, (2005) (PubMed).

Koseki, Miura, Fujimori, Hokari, Komoto, Hara, Ogino, Nagata, Goto, Hachimura, Kaminogawa, Ishii: "In situ demonstration of intraepithelial lymphocyte adhesion to villus microvessels of the small intestine." in: International immunology, Vol. 13, Issue 9, pp. 1165-74, (2001) (PubMed).

Papayannopoulou, Priestley, Nakamoto, Zafiropoulos, Scott, Harlan: "Synergistic mobilization of hemopoietic progenitor cells using concurrent beta1 and beta2 integrin blockade or beta2-deficient mice." in: Blood, Vol. 97, Issue 5, pp. 1282-8, (2001) (PubMed).

Sanchez-Madrid, Davignon, Martz, Springer: "Antigens involved in mouse cytolytic T-lymphocyte (CTL)-mediated killing: functional screening and topographic relationship." in: Cellular immunology, Vol. 73, Issue 1, pp. 1-11, (1983) (PubMed).

Details zu ITGAL

Target: ITGAL (Integrin alpha-L (ITGAL))

Andere Bezeichnung: CD11a (ITGAL Produkte)

Synonyme: CD11A antikoerper, LFA-1 antikoerper, LFA1A antikoerper, (p180) antikoerper, Cd11a antikoerper, LFA-1A antikoerper, Ly-15 antikoerper, Ly-21 antikoerper, CD11a antikoerper, ITGAL antikoerper, integrin subunit alpha L antikoerper, integrin alpha L antikoerper, ITGAL antikoerper, Itgal antikoerper

Hintergrund: Integrin subunit alpha L,CD11a (LFA-1 alpha) together with CD18 constitute leukocyte function-associated antigen 1 (LFA-1), the alphaLbeta2 integrin. CD11a is implicated in activation of LFA-1 complex. LFA-1 is expressed on the plasma membrane of leukocytes in a low-affinity conformation. Cell stimulation by chemokines or other signals leads to induction the high-affinity conformation, which supports tight binding of LFA-1 to its ligands, the intercellular adhesion molecules ICAM-1, -2, -3. LFA-1 is thus involved in interaction of various immune cells and in their tissue-specific settlement, but participates also in control of cell differentiation and proliferation and of T-cell effector functions. Blocking of LFA-1 function by specific antibodies or small molecules has become an important therapeutic approach in treatment of multiple inflammatory diseases. For example, humanized anti-LFA-1 antibody Efalizumab (Raptiva) is being used to interfere with T cell migration to sites of inflammation, binding of cholesterol-lowering drug simvastatin to CD11a allosteric site leads to immunomodulation and increase in lymphocytic cholinergic activity.,LFA-1, LFA1A, ITGAL

Gen-ID: 16408

UniProt: E9Q5M7

Pathways: Activated T Cell Proliferation, Integrin Complex