Blue Fluorescent Protein (BFP) Antikörper (Abberior STAR 580)

Details zu Produkt Nr. ABIN4368355, Anbieter: Anmelden zum Anzeigen
Antigen
Reaktivität
Entacmaea quadricolor
11
1
1
Wirt
Camelid (Camelidae)
11
2
Antikörpertyp
Recombinant Antibody
Klonalität (Klon)
Monoklonal ()
Konjugat
Abberior STAR 580
2
2
2
2
2
1
Applikation
Immunocytochemistry (ICC), Immunofluorescence (IF)
12
11
2
1
Optionen
Hersteller
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Hersteller Produkt- Nr.
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Verwendungszweck FluoTag-Q series - high affinity monovalent binders designed for quantitative imaging
Marke FluoTag-Q
Immunogen BFP
Klon 1H7
Fragment single-domain Antibody (sdAb)
Spezifität Specific for mTagBFP, mTagRFP and TagRFP657
Kreuzreaktivität (Details) Does not cross-react with common GFP- or dsRed/RFP derivatives
Produktmerkmale FluoTag-Q anti-BFP is derived from an in-house developed single-domain antibody (sdAb) that recognizes Blue Fluorescent Protein (BFP/TagBFP from Evrogen) with high affinity and specificity. In our FluoTag-Q series, each fluorophore is coupled to exactly one FluoTag, which in turn binds to its target molecule in a monovalent fashion. The high binding affinity and a high coupling efficiency of > 95 % guarantees a highly linear relation between target molecule number and fluorescent intensity. This enables you to directly count your target molecule of interest. The fluorophore is located exceptionally close to the recognized epitope (< 1.5 nm), which is ideal for all microscopy techniques.
Hintergrund Blue fluorescent protein BFP and its derivates have become very popular and universal tools in cell biology. It is a monomeric protein and an excellent fusion partner for chimeric proteins. The possibility to fuse BFP to the N- or the C-terminus of the protein of interest enables a wide range of application. However for some microscopy technics (like STROM or STED) more photostable or blinking fluorophores are needed, but also in conventional immnuofluorescence studies an amplification of the signal or the possible to image at another wavelength might be preferable. With our FluoTags the potential of BFP fusion proteins can be exceeded by far.
Molekulargewicht 26 kDa
Forschungsgebiet Tags/Labels
Applikationshinweise 1:1000 (IF, ICC)
Beschränkungen Nur für Forschungszwecke einsetzbar
Format Liquid
Konzentration 5 μM
Buffer PBS, 50 % glycerol, 0.09 % sodium azide
Konservierungsmittel Sodium azide
Vorsichtsmaßnahmen This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handhabung Protect from light!
Lagerung -20 °C
Informationen zur Lagerung Up to 3 months: -20 °C Up to 12 months: -80 °C or below
Bilder des Herstellers
Immunofluorescence (IF) image for anti-Blue Fluorescent Protein (BFP) antibody (Abberior STAR 580) (ABIN4368355) 3T3 cells transfected with the mitochondrial marker TOM70-mTagBFP (A, blue). Cells we...
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