RFP Antikörper (Red Fluorescent Protein) (Atto 647N)

Details for Product anti-RFP Antibody No. ABIN4368341, Anbieter: Anmelden zum Anzeigen
Antigen
  • RFP
  • RNF76
  • tripartite motif containing 27
  • TRIM27
Reaktivität
Discosoma
81
50
37
9
7
1
1
Wirt
Camelid (Camelidae)
94
38
13
9
1
Antikörpertyp
Recombinant Antibody
Klonalität (Klon)
Monoklonal ()
Konjugat
Dieser RFP Antikörper ist konjugiert mit Atto 647N
9
7
6
3
3
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
Applikation
Immunocytochemistry (ICC), Immunofluorescence (IF)
133
68
59
52
32
27
6
5
4
2
2
1
1
1
1
1
1
Optionen
Hersteller
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Hersteller Produkt- Nr.
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Verwendungszweck FluoTag-X series - Multiple fluorophores per affinity probe and multiple affinity probes per target molecule resulte in maximal brightness
Marke FluoTag-X
Immunogen RFP
Klon 2B12-2A1
Fragment single-domain Antibody (sdAb)
Spezifität Most common red fluorescent proteins like mRFP, mCherry, dsRed, tdTomato
Kreuzreaktivität (Details) Does not cross-react with GFP, mTagBFP or mTagRFP or their most common derivatives
Produktmerkmale FluoTag-X4 anti-RFP is a blend of two in-house developed single-domain antibodies (sdAbs) recognizing the most common red fluorescent proteins like mRFP, mCherry, DsRed and other DsRed derivatives with high affinity and specificity. In our FluoTag-X4 series, two fluorophore molecules are site-specifically coupled to each individual FluoTag molecule. The reagent therefore simultaneously targets up to four fluorophores to the protein of interest, which ensures extra-bright signals. Owing to the small size of our FluoTags, the distance between the target epitope and each fluorophore is below 4 nm. In comparison to conventional detection systems using conventional antibodies, our FluoTag-X4 series can thus improve the localization accuracy by 10-15 nm. Both features - superior brightness and precise fluorophore placement - render our FluoTag-X4 products excellent tools for all microscopy techniques.
Andere Bezeichnung RFP (RFP Antibody Abstract)
Hintergrund Red fluorescent protein RFP and its derivates have become very popular and universal tools in cell biology. It is a monomeric protein and an excellent fusion partner for chimeric proteins. The possibility to fuse RFP to the N- or the C-terminus of the protein of interest enables a wide range of application. However for some microscopy technics (like STROM or STED) more photostable or blinking fluorophores are needed, but also in conventional immnuofluorescence studies an amplification of the signal or the possible to image at another wavelength might be preferable. With our FluoTags the potential of RFP fusion proteins can be exceeded by far.
Molekulargewicht 26 kDa
Applikationshinweise 1:250 (IF,ICC)
Beschränkungen Nur für Forschungszwecke einsetzbar
Format Liquid
Konzentration 2.5 μM
Buffer PBS, 50 % glycerol, 0.09 % sodium azide
Konservierungsmittel Sodium azide
Vorsichtsmaßnahmen This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handhabung Protect from light!
Lagerung -20 °C
Informationen zur Lagerung Up to 3 months: -20 °C Up to 12 months: -80 °C or below
Bilder des Herstellers
Immunofluorescence (IF) image for anti-Red Fluorescent Protein (RFP) antibody (Atto 647N) (ABIN4368341) 3T3 cells transfected with the mitochondrial marker TOM70-mCherry (A, false color ill...
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