Rekombinanter RFP Antikörper (Atto 488)

Produktnummer ABIN4368335

Produktdetails anti-RFP Antikörper (Atto 488)

Target: RFP (Red Fluorescent Protein (RFP))

Antikörpertyp: Recombinant Antibody

Fragment: single-domain Antibody (sdAb)

Reaktivität: Discosoma

Wirt: Alpaca

Expressionssystem: E.coli

Klonalität: Monoklonal

Konjugat: Dieser RFP Antikörper ist konjugiert mit Atto 488

Applikation: Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Verwendungszweck: Camelid sdAb anti-RFP conjugated with Atto488, Clones 2B12/2A1

Spezifität: Recognizes most common red fluorescent proteins like mRFP and derivatives like mCherry, mScarlet-i, tdTomato, dsRed and mOrange.

Kreuzreaktivität (Details): Does not cross-react with GFP or mTagBFP derivatives.

Produktmerkmale: A dye conjugated alpaca single-domain antibody (sdAb), also referred to as VHH or nanobody. It is in the range of 15 kDa and 3 nm in molecular weight and size respectively. This means that sdAbs are ten times lighter and up to 5x smaller than a conventional IgG molecule. They can position a fluorophore up to 20 nm closer to the intended target than using conventional primary-secondary antibody complex detection.

Aufreinigung: Produced in: E.coli

Immunogen: RFP

Klon: 2B12-2A1

Labeling Ratio: A blend of 2 different sdAbs that bind at different epitopes of the same target protein, each sdAb bears 2 site-specifically coupled fluorophores.

Anwendungsinformationen

Applikationshinweise: Recommended dilution 1:500

Kommentare:

A blend of 2 different sdAbs that bind at different epitopes of the same target protein, two fluorophore molecules are site-specifically coupled to each individual sdAb molecule. The reagent therefore simultaneously targets up to four fluorophores to the protein of interest, which ensures extra-bright signals. Owing to the small size of sdAb, the distance between the target epitope and each fluorophore is below 4 nm. In comparison to conventional detection systems using conventional antibodies, this product can thus improve the localization accuracy by 10-15 nm. Both features - superior brightness and precise fluorophore placement - render this products excellent tools for all microscopy techniques.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Konzentration: 5 μM

Buffer: 1.25 μM of each fluorescently labeled sdAb clone in buffered saline, 50 % glycerol, 0.09 % sodium azide. 5 μM total fluorophore concentration

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Protect from light!

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: Up to 3 months store at -20 °C. Up to 12 months store at -80 °C or below

Haltbarkeit: 12 months

Details zu RFP

Target: RFP (Red Fluorescent Protein (RFP))

Andere Bezeichnung: RFP (RFP Produkte)

Molekulargewicht: 26 kDa