KRT1 Antikörper (Cytokeratin 1)

Details for Product anti-KRT1 Antibody No. ABIN4302150, Anbieter: Anmelden zum Anzeigen
Antigen
  • CK1
  • EHK
  • EHK1
  • EPPK
  • K1
  • KRT1A
  • NEPPK
  • Krt-2.1
  • Krt2-1
  • Krt86
  • KRT1
  • Kb1
  • KER1
  • CK-1
  • Keratin-1
  • EpK
  • cytok
  • cytokeratin
  • epi-keratin
  • epiker
  • xK81
  • keratin 1
  • Keratin, type I cytoskeletal 47 kDa L homeolog
  • KRT1
  • Krt1
  • xk81a1.L
Reaktivität
Huhn, Rind (Kuh), Hund, Human, Maus, Primate, Kaninchen, Ratte (Rattus)
508
198
174
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94
80
73
71
70
62
62
57
35
31
5
4
4
3
3
3
2
1
1
1
1
1
1
1
1
1
Wirt
Maus
468
64
8
Klonalität (Klon)
Monoklonal ()
Konjugat
Dieser KRT1 Antikörper ist unkonjugiert
25
24
19
19
18
17
17
17
17
17
17
17
17
16
16
15
15
9
2
1
Applikation
Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
397
252
241
166
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156
133
78
25
12
11
2
2
2
2
1
1
Optionen
Hersteller
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Hersteller Produkt- Nr.
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Immunogen Total keratin isolated from human epidermal callus was used as immunogen to generate the pan cytokeratin antibodies AE1 + AE3 (Woodcock-Mitchell, 1982).
Klon AE1-AE3
Isotyp IgG1 kappa
Spezifität Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI <5.7) and basic (pI >6.0) subfamilies. This antibody cocktail recognizes acidic (Type I or LMW) and basic (Type II or HMW) cytokeratins, which include CK1, CK3, CK4, CK5, CK6, CK8, CK10, CK14, CK15, CK16, and CK19. Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis. AE-1/AE-3 is a broad spectrum anti pan-cytokeratin antibody cocktail, which differentiates epithelial tumors from non-epithelial tumors e.g. squamous vs. adenocarcinoma of the lung, liver carcinoma, breast cancer, and esophageal cancer. It has been used to characterize the source of various neoplasms and to study the distribution of cytokeratin containing cells in epithelia during normal development and during the development of epithelial neoplasms. This antibody stains cytokeratins present in normal and abnormal human tissues and has shown high sensitivity in the recognition of epithelial cells and carcinomas.
Reinigung Protein A or G purified
Andere Bezeichnung Cytokeratin (KRT1 Antibody Abstract)
Hintergrund Gene Symbol: KRT1
Gen-ID 3848
Applikationshinweise Western Blot 0.5-1 μg/mL, Flow Cytometry 0.5-1 μg/million cells, Immunocytochemistry/Immunofluorescence 1-2 μg/mL, Immunohistochemistry-Paraffin 0.5-1.0 μg/mL, Immunohistochemistry-Frozen 0.5-1.0 μg/mLMol. Weight of Antigen: 40 - 67 kDa 1. The staining pattern of the pan cytokeratin antibody cocktail may be different than that of either antibody separately. 2. The pan cytokeratin cocktail does not react with keratin18, which is also expressed in carcinomas. As such, negative staining with NBP2-29429 in of itself may not be sufficient evidence to rule out the possibility of a carcinoma (Ordonez, 2013). a. For example, hepatocellular, adrenal cortical, clear cell renal and chromophobe renal cell carcinomas have been reported to be negative for the pan cytokeratin antibody. In this regard, the pan cytokeratin antibody can be used as part of a screening panel to more extensively define the tumor cell lineages. 3. The pan cytokeratin antibody may cross-react with GFAP, leading to aberrant positive staining of glial tumors such as ependymoma, glioblastoma, or schwannoma (Ordonez, 2013).
Kommentare

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Beschränkungen Nur für Forschungszwecke einsetzbar
Format Liquid
Buffer 10 mM PBS and 0.05 % BSA
Buffer contains: 0.05 % Sodium Azide
Konservierungsmittel Sodium azide
Vorsichtsmaßnahmen This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung 4 °C
Informationen zur Lagerung Store at 4°C.
Bilder des Herstellers
Flow Cytometry (FACS) image for anti-Cytokeratin 1 (KRT1) antibody (ABIN4302150) Flow Cytometry: pan Cytokeratin Antibody (AE1 + AE3) [NBP2-29429] - Analysis using Az...
Flow Cytometry (FACS) image for anti-Cytokeratin 1 (KRT1) antibody (ABIN4302150) Flow Cytometry: pan Cytokeratin Antibody (AE1 + AE3) [NBP2-29429] - Human Pan-Cytoker...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-Cytokeratin 1 (KRT1) antibody (ABIN4302150) Immunohistochemistry-Paraffin: pan Cytokeratin Antibody (AE1 + AE3) [NBP2-29429] - Fo...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-Cytokeratin 1 (KRT1) antibody (ABIN4302150) Immunohistochemistry-Paraffin: pan Cytokeratin Antibody (AE1 + AE3) [NBP2-29429] - An...
Immunofluorescence (IF) image for anti-Cytokeratin 1 (KRT1) antibody (ABIN4302150) Immunocytochemistry/Immunofluorescence: pan Cytokeratin Antibody (AE1 + AE3) [NBP2-29...
Flow Cytometry (FACS) image for anti-Cytokeratin 1 (KRT1) antibody (ABIN4302150) Flow Cytometry: pan Cytokeratin Antibody (AE1 + AE3) - Using the PE direct conjugate...
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) image for anti-Cytokeratin 1 (KRT1) antibody (ABIN4302150) Immunohistochemistry-Paraffin: pan Cytokeratin Antibody (AE1 + AE3) - Pan Cytokerati...
Produkt verwendet in: Ordóñez: "Broad-spectrum immunohistochemical epithelial markers: a review." in: Human pathology, Vol. 44, Issue 7, pp. 1195-215, 2013 (PubMed). Weitere Details: Immunohistochemistry (Paraffin-embedded Sections)

Berg, Berner, Lilleby, Bruland, Fosså, Nesland, Kvalheim: "Impact of disseminated tumor cells in bone marrow at diagnosis in patients with nonmetastatic prostate cancer treated by definitive radiotherapy." in: International journal of cancer, Vol. 120, Issue 8, pp. 1603-9, 2007 (PubMed). Weitere Details: Immunocytochemistry,Immunofluorescence

Niikura, Okamoto, Yoshinaga, Nagase, Takano, Ito, Yaegashi: "Detection of micrometastases in the sentinel lymph nodes of patients with endometrial cancer." in: Gynecologic oncology, Vol. 105, Issue 3, pp. 683-6, 2007 (PubMed). Weitere Details: Immunohistochemistry (Paraffin-embedded Sections)

Azumi, Saga, Hashimoto, Kakizaki: "[Histological investigation of prostate cancer treated with hormonal agents]." in: Hinyokika kiyo. Acta urologica Japonica, Vol. 52, Issue 10, pp. 781-4, 2006 (PubMed).

Alexander-Sefre, Singh, Ayhan, Thomas, Jacobs: "Assessment of the depth of myometrial invasion in stage I endometrioid endometrial cancer using pancytokeratin immunohistochemistry." in: International journal of gynecological cancer : official journal of the International Gynecological Cancer Society, Vol. 14, Issue 4, pp. 665-72, 2004 (PubMed). Weitere Details: Immunohistochemistry (Paraffin-embedded Sections)

Wiedswang, Borgen, Kåresen, Qvist, Janbu, Kvalheim, Nesland, Naume: "Isolated tumor cells in bone marrow three years after diagnosis in disease-free breast cancer patients predict unfavorable clinical outcome." in: Clinical cancer research : an official journal of the American Association for Cancer Research, Vol. 10, Issue 16, pp. 5342-8, 2004 (PubMed).

Krishnamurthy, Sneige, Ordóñez, Hunt, Kuerer: "Characterization of foam cells in nipple aspirate fluid." in: Diagnostic cytopathology, Vol. 27, Issue 5, pp. 261-4; discussion 265, 2002 (PubMed). Weitere Details: Immunocytochemistry

Alibardi, Maurizii, Taddei: "Immunocytochemical and electrophoretic distribution of cytokeratins in the resting stage epidermis of the lizard Podarcis sicula." in: The Journal of experimental zoology, Vol. 289, Issue 7, pp. 409-18, 2001 (PubMed). (Probematerial (Species): Lizard). Weitere Details: Immunocytochemistry,Western Blotting,Immunofluorescence

Woodcock-Mitchell, Eichner, Nelson, Sun: "Immunolocalization of keratin polypeptides in human epidermis using monoclonal antibodies." in: The Journal of cell biology, Vol. 95, Issue 2 Pt 1, pp. 580-8, 1983 (PubMed). (Probematerial (Species): Human). Weitere Details: Western Blotting

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