PPP1R13L Antikörper (Isoform 1)

Produktnummer ABIN401339

Produktdetails anti-PPP1R13L Antikörper

Target: PPP1R13L (Protein Phosphatase 1, Regulatory Subunit 13 Like (PPP1R13L))

Bindungsspezifität: AA 780-797, Isoform 1

Reaktivität: Human, Maus, Hund

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser PPP1R13L Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Spezifität: This antibody is directed against the iASPP protein. Expect bands approximately 100 kDa and 50 kDa in size corresponding to Isoforms 1 and 2 respectively of iASPP protein by Western blotting in the appropriate cell lysate or extract. Partial reactivity may occur against iASPP from Bovine.

Kreuzreaktivität (Details): Species reactivity (expected):Mouse, Rat, Canine.
Species reactivity (tested):Human.

Aufreinigung: Affinity Chromatography.

Immunogen: Synthetic peptide corresponding to amino acids 780-797 of Human iASPP (isoform 1) protein

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Suitable for use in ELISA, Immunohistochemistry and Western blot. Recommended Dilutions: ELISA: 1/40,000-1/160,000. Western Blot: 1/1,000-1/5,000. Immunohistochemistry: 1/1,000-1/5,000.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.19 mg/mL (by UV absorbance at 280 nm)

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 0.01 % (w/v) Sodium Azide as preservative.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid cycles of freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer. Dilute only prior to immediate use.

Details zu PPP1R13L

Target: PPP1R13L (Protein Phosphatase 1, Regulatory Subunit 13 Like (PPP1R13L))

Andere Bezeichnung: PPP1R13L (PPP1R13L Produkte)

Synonyme: AV260376 antikoerper, IASPP antikoerper, Nkip1 antikoerper, wa3 antikoerper, zgc:113058 antikoerper, NKIP1 antikoerper, RAI antikoerper, RAI4 antikoerper, protein phosphatase 1, regulatory (inhibitor) subunit 13 like antikoerper, protein phosphatase 1, regulatory subunit 13 like antikoerper, protein phosphatase 1 regulatory subunit 13 like antikoerper, protein phosphatase 1 regulatory subunit 13 like L homeolog antikoerper, Ppp1r13l antikoerper, ppp1r13l antikoerper, PPP1R13L antikoerper, ppp1r13l.L antikoerper

Hintergrund: ASPP proteins (ASPP1, ASPP2 and iASPP) represent a new family of p53 binding proteins. ASPP1 and ASPP2 bind and enhance p53-mediated apoptosis. In contrast, the third member, iASPP, functionally inactivates p53. iASPP (also called protein phosphatase 1 regulatory (inhibitor) subunit 13 like protein, Inhibitor of ASPP protein, Protein iASPP, PPP1R13B-like protein and NFkappaB-interacting protein 1) plays a central role in regulation of apoptosis and transcription via its interaction with NF-kappa-B and p53/TP53 proteins. iASPP blocks transcription of HIV-1 virus by inhibiting the action of both NF-kappa-B and SP1. This protein also inhibits p53/TP53 function, possibly by preventing the association between p53/TP53 and ASPP1 or ASPP2, and therefore suppressing the subsequent activation of apoptosis. iASPP is predominantly a cytoplasmic protein (isoform 1) but can also be found in the nucleus (isoform 2). iASPP is highly expressed in heart, placenta and prostate and is weakly expressed in brain, liver, skeletal muscle, testis and peripheral blood leukocyte. The N-terminal region of isoform 1 is required for cytoplasmic localization. Defects in iASPP may be a cause of certain breast cancers and the protein is overexpressed in many patients suffering from breast carcinomas and expressing a wild-type p53/TP53 protein.Synonyms: IASPP, Inhibitor of ASPP protein, NFkB-interacting protein 1, NKIP1, PPP1R13B-like protein, PPP1R13BL, RAI, RelA-associated inhibitor

Gen-ID: 10848

NCBI Accession: NP_001135974

UniProt: Q8WUF5