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V5 Epitope Tag Antikörper

WB, EIA Wirt: Kaninchen Polyclonal unconjugated
Produktnummer ABIN401225
  • Target Alle V5 Epitope Tag Produkte
    V5 Epitope Tag
    Reaktivität
    Bitte anfragen
    Wirt
    • 60
    • 41
    • 10
    • 7
    • 6
    • 2
    • 1
    Kaninchen
    Klonalität
    • 57
    • 57
    • 10
    Polyklonal
    Konjugat
    • 66
    • 7
    • 6
    • 5
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Dieser V5 Epitope Tag Antikörper ist unkonjugiert
    Applikation
    • 101
    • 59
    • 44
    • 44
    • 31
    • 30
    • 13
    • 10
    • 8
    • 6
    • 6
    • 6
    • 6
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), Enzyme Immunoassay (EIA)
    Sequenz
    GKPIPNPLLG LDST
    Aufreinigung
    Affinity chromatography
    Immunogen
    Peptide corresponding to aa 95-108 of the V protein conjugated to KLH using maleimide
    Isotyp
    IgG
  • Applikationshinweise
    ELISA: 1: 10,000 - 1: 60,000. Western blot: 1: 5,000 - 1: 10,000. Immunohistochemistry: 1: 500 - 1: 3,000.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Konzentration
    1.0 mg/mL (by UV absorbance at 280 nm)
    Buffer
    0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01 % (w/v) Sodium Azide
    Konservierungsmittel
    Sodium azide
    Vorsichtsmaßnahmen
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handhabung
    Avoid repeated freezing and thawing. Should this product contain a precipitate we recommend microcentrifugation before use.
    Lagerung
    4 °C/-20 °C
    Informationen zur Lagerung
    Store the antibody at 2 - 8 °C up to one month or for longer (in aliquots) at -20 °C or below.
  • Target
    V5 Epitope Tag
    Andere Bezeichnung
    Pk (V5) Epitope Tag (GKPIPNPLLGLDST) (V5 Epitope Tag Produkte)
    Substanzklasse
    Tag
    Hintergrund
    Epitope tags are short peptide sequences that are easily recognized by tag-specific antibodies. Due to their small size, epitope tags do not affect the tagged protein's biochemical properties. Most often sequences encoding the epitope tag are included with target DNA at the time of cloning to produce fusion proteins containing the epitope tag sequence. This allows anti-epitope tag antibodies to serve as universal detection reagents for any tag containing protein produced by recombinant means. This means that anti-epitope tag antibodies are a useful alternative to generating specific antibodies to identify, immunoprecipitate or immunoaffinity purify a recombinant protein.
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