V5 Epitope Tag Antikörper
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- Target Alle V5 Epitope Tag Produkte
- V5 Epitope Tag
- Reaktivität
- Bitte anfragen
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Wirt
- Kaninchen
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Klonalität
- Polyklonal
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Konjugat
- Dieser V5 Epitope Tag Antikörper ist unkonjugiert
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Applikation
- Western Blotting (WB), Enzyme Immunoassay (EIA)
- Sequenz
- GKPIPNPLLG LDST
- Aufreinigung
- Affinity chromatography
- Immunogen
- Peptide corresponding to aa 95-108 of the V protein conjugated to KLH using maleimide
- Isotyp
- IgG
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- Applikationshinweise
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ELISA: 1: 10,000 - 1: 60,000. Western blot: 1: 5,000 - 1: 10,000. Immunohistochemistry: 1: 500 - 1: 3,000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Konzentration
- 1.0 mg/mL (by UV absorbance at 280 nm)
- Buffer
- 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01 % (w/v) Sodium Azide
- Konservierungsmittel
- Sodium azide
- Vorsichtsmaßnahmen
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handhabung
- Avoid repeated freezing and thawing. Should this product contain a precipitate we recommend microcentrifugation before use.
- Lagerung
- 4 °C/-20 °C
- Informationen zur Lagerung
- Store the antibody at 2 - 8 °C up to one month or for longer (in aliquots) at -20 °C or below.
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- Target
- V5 Epitope Tag
- Andere Bezeichnung
- Pk (V5) Epitope Tag (GKPIPNPLLGLDST) (V5 Epitope Tag Produkte)
- Substanzklasse
- Tag
- Hintergrund
- Epitope tags are short peptide sequences that are easily recognized by tag-specific antibodies. Due to their small size, epitope tags do not affect the tagged protein's biochemical properties. Most often sequences encoding the epitope tag are included with target DNA at the time of cloning to produce fusion proteins containing the epitope tag sequence. This allows anti-epitope tag antibodies to serve as universal detection reagents for any tag containing protein produced by recombinant means. This means that anti-epitope tag antibodies are a useful alternative to generating specific antibodies to identify, immunoprecipitate or immunoaffinity purify a recombinant protein.
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