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GST Antikörper (HRP)

GST Reaktivität: Schistosoma japonicum WB, ELISA Wirt: Ziege Polyclonal HRP
Produktnummer ABIN398845
  • Target Alle GST Antikörper anzeigen
    GST (Glutathione S Transferase (GST))
    Reaktivität
    • 77
    • 33
    • 31
    • 11
    • 4
    • 3
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Schistosoma japonicum
    Wirt
    • 63
    • 59
    • 43
    • 2
    • 2
    • 1
    Ziege
    Klonalität
    • 102
    • 66
    Polyklonal
    Konjugat
    • 75
    • 13
    • 11
    • 10
    • 5
    • 5
    • 4
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Dieser GST Antikörper ist konjugiert mit HRP
    Applikation
    • 141
    • 75
    • 36
    • 29
    • 22
    • 17
    • 16
    • 15
    • 10
    • 8
    • 8
    • 7
    • 6
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), ELISA
    Spezifität
    Goat Anti-GST-tag [HRP] Polyclonal Antibody detects GST fusion proteins in expression systems including bacterial expression system, mammalian expression system and others.
    Aufreinigung
    Immunoaffinity chromatography
    Immunogen
    Recombinant Glutathione S-Transferase (GST) from Schistosoma japonicum.
    Isotyp
    IgG
    Top Product
    Discover our top product GST Primärantikörper
  • Applikationshinweise
    Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.

    ELISA: 0.05-0.2 µg/mL Western blot: 0.2-1 µg/mLOther Applications: user-optimized
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Lyophilized
    Rekonstitution
    Reconstitute the lyophilized powder with deionized water (or equivalent) to a final concentration of 0.5 mg/mL.
    Buffer
    PBS, pH 7.4, containing 1 % BSA and 0.01 % thimerosal.
    Konservierungsmittel
    Thimerosal (Merthiolate)
    Vorsichtsmaßnahmen
    This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Lagerung
    4 °C/-20 °C
    Informationen zur Lagerung
    The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.
  • Savar, Jahanian-Najafabadi, Mahdavi, Shokrgozar, Jafari, Bouzari et al.: "In silico and in vivo studies of truncated forms of flagellin (FliC) of enteroaggregative Escherichia coli fused to FimH from uropathogenic Escherichia coli as a vaccine candidate against urinary ..." in: Journal of biotechnology, Vol. 175, pp. 31-7, (2014) (PubMed).

    Gish, Gagne, Han, Deyoung, Clark: "WUSCHEL-responsive At5g65480 interacts with CLAVATA components in vitro and in transient expression." in: PLoS ONE, Vol. 8, Issue 6, pp. e66345, (2013) (PubMed).

  • Target
    GST (Glutathione S Transferase (GST))
    Andere Bezeichnung
    Glutathione-S-Transferase (GST) (GST Produkte)
    Synonyme
    GST antikoerper, GST 13-13 antikoerper, GST13 antikoerper, GST13-13 antikoerper, GSTK1-1 antikoerper, hGSTK1 antikoerper, 1500002K10Rik antikoerper, Gst antikoerper, Ptgds2 antikoerper, glutathione S-transferase kappa 1 antikoerper, microsomal glutathione S-transferase 1 antikoerper, hematopoietic prostaglandin D synthase antikoerper, GSTK1 antikoerper, Mgst1 antikoerper, Hpgds antikoerper
    Hintergrund
    Schistosoma japonicum glutathione S-transferase (GST) is a 26,000 Da enzyme that conjugates reduced glutathione to hydrophobic electrophiles. GST is an ideal fusion partner for production of foreign proteins in commercially available expression systems because it can be expressed in high levels in E. coli. The fusion proteins can be purified to homogeneity in a single step as the GST portion of the protein binds tightly to immobilized glutathione. Elution can be accomplished by using free glutathione. The GST portion of the fusion protein can be separated from the protein of interest by introducing site-specific proteases. Antibodies directed against GST can detect both the fusion protein during purification and the cleavage of GST from the protein of interest.Goat Anti-GST-tag [HRP] Polyclonal Antibody is developed in goat using a recombinant Glutathione S-Transferase (GST) from Schistosoma japonicum. The antibody is highly purified by immunoaffinity chromatography from goat antiserum and is then conjugated to horseradish peroxidase.
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