Ziege anti-Ratte IgG (Heavy & Light Chain) Antikörper (HRP)

Produktnummer ABIN398396

Produktdetails

Target: IgG

Bindungsspezifität: Heavy & Light Chain

Reaktivität: Ratte

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: HRP

Applikation: ELISA, Western Blotting (WB)

Produktmerkmale: This polyclonal antibody is highly purified from goat antiserum by immunoaffinity chromatography and then conjugated to horseradish peroxidase. It reacts with rat IgG heavy and light chains.

Aufreinigung: Immunoaffinity chromatography

Immunogen: Full-length rat IgG (purified)

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Antibodies-Online Goat Anti-Rat IgG (H&L) [HRP] is suitable for immunoblotting (Western and Dot blots), ELISA, immunoperoxidase electron microscopy, immunohistochemistry, and other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.The investigator must determine the ideal working concentration for each specific application. The ideal working concentration must take into account such factors as secondary antibody affinity, antigen concentration, sensitivity of the detection method, temperature, and the length of the incubations. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.ELISA: 1:5,000-1:50,000
Western blot: 1:1,000-1:5,000Other applications: user-optimized.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: PBS, pH 7.4, containing 1 % BSA, and 0.01 % thimerosal

Konservierungsmittel: Thimerosal (Merthiolate)

Vorsichtsmaßnahmen: Biohazard Informations: This product contains thimerosal which is hazardous.

Handhabung: Small volume of antibody will occasionally become entrapped in the cap of the vial during shipment and storage. Before use, centrifuge the vial briefly to bring down any liquid in the cap.
Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: The antibody is stable for 2-3 weeks if stored at 2-8 °C. For long term storage, aliquot and store at -28 °C or below. Avoid repeated freezing and thawing cycles.

Referenzen

Deckert, Kretz, Habbout, Raghay, Labbé, Abello, Desrumaux, Gautier, Lemaire-Ewing, Maquart, Le Guern, Masson, Steinmetz, Lagrost: "Development of abdominal aortic aneurysm is decreased in mice with plasma phospholipid transfer protein deficiency." in: The American journal of pathology, Vol. 183, Issue 3, pp. 975-86, (2013) (PubMed).

Antigendetails

Target: IgG

Abstract: IgG Produkte

Substanzklasse: Antibody