Ziege anti-Maus IgG (Heavy & Light Chain) Antikörper (HRP) Secondary Antibody
- Target See all IgG products
- Heavy & Light Chain
- ELISA, Western Blotting (WB)
- This polyclonal antibody is highly purified from goat antiserum by immunoaffinity chromatography and then conjugated to horseradish peroxidase. It reacts with mouse IgG heavy and light chains.
- Immunoaffinity chromatography
- Full length mouse IgG (purified)
Antibodies-Online Goat Anti-Mouse IgG (H&L) [HRP] is suitable for immunoblotting (western and dot blots), ELISA, immunoperoxidase electron microscopy, immunohistochemistry, and other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.The investigator must determine the ideal working concentration for each specific application. The ideal working concentration must take into account such factors as secondary antibody affinity, antigen concentration, sensitivity of the detection method, temperature, and the length of the incubations. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.ELISA: 1:5,000-1:20,000
Western blot: 1:1,000-1:10,000Other applications: user-optimized
- Nur für Forschungszwecke einsetzbar
- Reconstitute the lyophilized antibody with deionized water (or equivalent) to a final concentration of 1.0 mg/ml.
- 1 mg/mL
- PBS, pH 7.4, containing 1 % BSA and 0.01 % Thimerosal
- Biohazard Informations: This product contains thimerosal which is hazardous.
Avoid repeated freezing and thawing. Dilute only prior to immediate use
Centrifuge product if not completely clear after standing at room temperature.
Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.
- 4 °C/-20 °C
- Informationen zur Lagerung
- Goat Anti-Mouse IgG (H&L) [HRP] Polyclonal Antibody is stable for 2-3 weeks at 2-8 °C. For long term storage, aliquot and store at -28 °C or below.
- 3 weeks
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