UBE2F Antikörper (C-Term)

Produktnummer ABIN375151

Produktdetails anti-UBE2F Antikörper

Target: UBE2F (Ubiquitin-Conjugating Enzyme E2F (UBE2F))

Bindungsspezifität: C-Term

Reaktivität: Human, Maus, Ratte, Rind (Kuh), Hund

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser UBE2F Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Sequenz: C-HLRDKEDFRN KVDD

Spezifität: This antibody recognizes NCE2 / UBE2F.

Kreuzreaktivität (Details): Species reactivity (expected):Rat, Canine (Dog), Bovine.
Species reactivity (tested):Human, Mouse.

Aufreinigung: Ammonium Sulphate Precipitation followed by antigen Affinity Chromatography using the immunizing peptide

Immunogen: Peptide with sequence from the C Terminus of the protein sequence according to NP_542409.1. Genename: UBE2F

Anwendungsinformationen

Applikationshinweise: Peptide ELISA: 1/32000. Western Blot: 0.05 - 0.2 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.5 mg/mL

Buffer: Tris buffered saline, pH ~7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Details zu UBE2F

Target: UBE2F (Ubiquitin-Conjugating Enzyme E2F (UBE2F))

Andere Bezeichnung: NCE2 (UBE2F Produkte)

Synonyme: NCE2 antikoerper, 2510010F15Rik antikoerper, AI851109 antikoerper, RGD1307608 antikoerper, zgc:77005 antikoerper, ubiquitin conjugating enzyme E2 F (putative) antikoerper, NEDD8-conjugating enzyme UBE2F antikoerper, ubiquitin-conjugating enzyme E2F (putative) antikoerper, ubiquitin conjugating enzyme E2F (putative) S homeolog antikoerper, UBE2F antikoerper, Tsp_01616 antikoerper, ube2f antikoerper, ube2f.S antikoerper, Ube2f antikoerper

Hintergrund: NCE2 is a ubiquitin conjugating enzyme. Ubiquitin is a 76 amino acid highly conserved eukaryotic polypeptide that selectively marks cellular proteins for proteolytic degradation by the 26S proteasome. The process of target selection, covalent attachment and shuttle to the 26S proteasome is a vital means of regulating the concentrations of key regulatory proteins in the cell by limiting their lifespans. Polyubiquitination is a common feature of this modification. Serial steps for modification include the activation of ubiquitin, an ATP dependent formation of a thioester bond between ubiquitin and the enzyme E1, transfer by transacylation of ubiquitin from E1 to the ubiquitin conjugating enzyme E2, and covalent linkage to the target protein directly by E2 or via E3 ligase enzyme. Deubiquitination enzymes also exist to reverse the marking of protein substrates. Posttranslational tagging by Ub is involved in a multitude of cellular processes, including the cell cycle, cell growth and differentiation, embryogenesis, apoptosis, signal transduction, DNA repair, regulation of transcription and DNA replication, transmembrane transport, stress responses, the immune response, and nervous system functions.Synonyms: NEDD8-conjugating enzyme 2, UBE2F, Ubiquitin-conjugating enzyme E2 F

Molekulargewicht: 21.1kDa (Human NP_542409.1 and Mouse NP_080730.1).

Gen-ID: 140739, 9606

UniProt: Q969M7