Maus anti-Human IgE (Fc Region) Antikörper

Details zu Produkt Nr. ABIN363704, Anbieter: Anmelden zum Anzeigen
Antigen
Epitop
Fc Region
168
53
52
7
1
1
1
1
1
Reaktivität
Human
495
152
51
38
6
6
3
2
2
2
1
1
1
Wirt
Maus
294
290
67
62
15
5
3
1
1
Klonalität (Klon)
Monoklonal ()
Applikation
Functional Studies (Func), Flow Cytometry (FACS), ELISA, Western Blotting (WB)
Optionen
Hersteller
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Hersteller Produkt- Nr.
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Immunogen purified human myeloma IgE
Klon BSW17-NBS01
Isotyp IgG1 kappa
Spezifität Human IgE, Fce3-4, no cross-ractivity with other human or murine Ab isotypes
Produktmerkmale IGHE, chromosome 14q32.33
Reinigung Affinity - purified from cell culture supernatant by Immunoaffinity Chromatography on CH-Sepharose 4B
Reinheit > 95 %, tested by analytical Gel Permeation Chromatography
Hintergrund The antibody recognizes a conformational epitope within the Ce3 domain of human IgE spanning towards the amino-terminal part of the Ce4 domain. It blocks the binding of IgE to its high affinity receptor on mast cells and basophils. It is therefore suited as an inhibitory reference compound for mast cell modulator profiling and basic research on IgE mediated mast cell receptor signaling.
Applikationshinweise When used for functional cellular assays (mast cell / basophil activation) at dilutions < 1:100, the Na-azide free formulation is recommended. Quality assurance: Reactivity > 99 %, compared to calibrator (Internal Standard mAb BSW17), tested by ELISA binding to immobilized human Standard IgE (SUS-11)
Beschränkungen Nur für Forschungszwecke einsetzbar
Konzentration 1 mg/mL
Buffer PBS, pH=7.2, 0.1 % NaN3
Konservierungsmittel Sodium azide
Vorsichtsmaßnahmen WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handhabung Monoclonal antibodies should not be stored at a temperature below -25 °C due to the aggregation effect of the protein.
Lagerung 4 °C
Bilder des Herstellers
 image for Mouse anti-Human IgE (Fc Region) antibody (ABIN363704) product purity: gel permeation chromatography (Superose 12/HR)
 image for Mouse anti-Human IgE (Fc Region) antibody (ABIN363704) Target binding kinetics (Biacore
Produkt verwendet in: Rudolf, Zuercher, Nechansky, Ruf, Vogel, Miescher, Stadler, Kricek: "Molecular basis for nonanaphylactogenicity of a monoclonal anti-IgE antibody." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 165, Issue 2, pp. 813-9, 2000 (PubMed).

Allgemeine Veröffentlichungen Rudolf, Furukawa, Miescher, Vogel, Kricek, Stadler: "Effect of anti-IgE antibodies on Fc epsilonRI-bound IgE." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 157, Issue 12, pp. 5646-52, 1997 (PubMed).

Czech, Stadler, Schuopf, Kapp: "IgE autoantibodies in atopic dermatitis--occurrence of different antibodies against the CH3 and the CH4 epitopes of IgE." in: Allergy, Vol. 50, Issue 3, pp. 243-8, 1995 (PubMed).

Knutti-Mueller, Stadler, Magnusson, de Weck: "Human IgE synthesis in vitro. Detection with monoclonal antibodies." in: Allergy, Vol. 41, Issue 6, pp. 457-67, 1987 (PubMed).