The antibody detects endogenous level of P38MAPK only when phosphorylated at tyrosine 182.
Aufreinigung
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Immunogen
Peptide sequence around phosphorylation site of pTyr182 (T-G-Y (p) -V-A) derived from Human P38 MAPK. Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates.
MAPK14
Reaktivität: Human
WB, IHC, IF, IP
Wirt: Kaninchen
Polyclonal
unconjugated
Applikationshinweise
Western blotting: 1:500-1:1000 Immunohistochemistry: 1:50-1:100
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Liquid
Konzentration
1 mg/mL
Buffer
Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
Konservierungsmittel
Sodium azide
Vorsichtsmaßnahmen
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung
4 °C/-20 °C
Informationen zur Lagerung
Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.
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Mashima, Sato, Sugimoto, Tsuruo, Seimiya: "Promotion of glioma cell survival by acyl-CoA synthetase 5 under extracellular acidosis conditions." in: Oncogene, Vol. 28, Issue 1, pp. 9-19, (2009) (PubMed).
Chen, Song, Zhou, Tong, Li, Fu, Guo, Dong, He, Qiao, Zhan, Li: "Disruption of xCT inhibits cancer cell metastasis via the caveolin-1/beta-catenin pathway." in: Oncogene, Vol. 28, Issue 4, pp. 599-609, (2009) (PubMed).
Target
MAPK14
(Mitogen-Activated Protein Kinase 14 (MAPK14))
Responds to activation by environmental stress, pro-inflammatory cytokines and lipopolysaccharide (LPS) by phosphorylating a number of transcription factors, such as ELK1 and ATF2 and several downstream kinases, such as MAPKAPK2 and MAPKAPK5. Plays a critical role in the production of some cytokines, for example IL-6. May play a role in stabilization of EPO mRNA during hypoxic stress. Isoform Mxi2 activation is stimulated by mitogens and oxidative stress and only poorly phosphorylates ELK1 and ATF2. Isoform Exip may play a role in the early onset of apoptosis.