Protein A column, followed by two-step phosphospecific peptide affinity purification
Immunogen
This antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S254 of human ENT1.
SLC29A1
Reaktivität: Human
WB, ELISA
Wirt: Kaninchen
Polyclonal
unconjugated
Applikationshinweise
ELISA: 1/1,000. Dot Blot: 1/500. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Liquid
Konzentration
0.25 mg/mL
Buffer
PBS with 0.09 % (W/V) sodium azide
Konservierungsmittel
Sodium azide
Vorsichtsmaßnahmen
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handhabung
Avoid repeated freezing and thawing.
Lagerung
4 °C/-20 °C
Informationen zur Lagerung
Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.
Dephoure, Zhou, Villén, Beausoleil, Bakalarski, Elledge, Gygi: "A quantitative atlas of mitotic phosphorylation." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 105, Issue 31, pp. 10762-7, (2008) (PubMed).
Target
SLC29A1
(Solute Carrier Family 29 (Nucleoside Transporters), Member 1 (SLC29A1))
ENT1 antikoerper, rENT1 antikoerper, 1200014D21Rik antikoerper, AA407560 antikoerper, solute carrier family 29 member 1 (Augustine blood group) antikoerper, solute carrier family 29 member 1 antikoerper, solute carrier family 29 (nucleoside transporters), member 1 antikoerper, SLC29A1 antikoerper, Slc29a1 antikoerper
Hintergrund
ENT1 is a member of the equilibrative nucleoside transporter family. The protein is categorized as an equilibrative (as opposed to concentrative) transporter that is sensitive to inhibition by nitrobenzylthioinosine (NBMPR). Nucleoside transporters are required for nucleotide synthesis in cells that lack de novo nucleoside synthesis pathways, and are also necessary for the uptake of cytotoxic nucleosides used for cancer and viral chemotherapies.Synonyms: Equilibrative nucleoside transporter 1, Solute carrier family 29 member 1