KIAA0319 Antikörper (AA 391-403)

Produktnummer ABIN342965

Produktdetails anti-KIAA0319 Antikörper

Target: KIAA0319

Bindungsspezifität: AA 391-403

Reaktivität: Maus, Ratte

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser KIAA0319 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA

Spezifität: Mouse KIAA0319. The variants XP_341528.2 and XP_001070349.1 represent identical protein. This antibody is expected to cross-react with mouse KIAA0319 isoform 1 (NP_001074520.1), isoform 2 (XP_994023.1) and isoform 4 (XP_913491.1).

Homologie: Percent identity by BLAST analysis: Mouse, Rat (100%) Hamster (85%).

Aufreinigung: Immunoaffinity purified

Immunogen: Synthetic peptide C-QGKIKQENKPTLH from an internal region of mouse KIAA0319 (NP_001074520.1). Percent identity by BLAST analysis: Mouse, Rat (100%), Hamster (85%).

Type of Immunogen: Synthetic peptide

Anwendungsinformationen

Applikationshinweise: Approved: Peptide-ELISA (1:128000, 0.5 - 1.5 μg/mL), WB (0.5 - 1.5 μg/mL)

Usage: Peptide ELISA: antibody detection limit dilution 1:128000. Western Blot: Approx 150kD band observed in Mouse Brain lysates (calculated MW of 118kD according to Mouse NP_001074520.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Velayos-Baeza, Hum Mol Genet. 2008 Mar 15,17(6):859-71, PMID: 18063668). Recommended concentration: 0.5-1.5 μg/mL. An additional band of unknown identity was also consistently observed at 25kD. This band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies / lysates? Have any further splice variants / modified forms been reported?

Kommentare:

Target Species of Antibody: Mouse

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Store at -20°C. Avoid freeze-thaw cycles.

Details zu KIAA0319

Target: KIAA0319

Andere Bezeichnung: KIAA0319 (KIAA0319 Produkte)

Synonyme: DYLX2 antikoerper, DYX2 antikoerper, 4930451E12Rik antikoerper, Kiaa0319 antikoerper, KIAA0319 antikoerper, RIKEN cDNA D130043K22 gene antikoerper, KIAA0319 ortholog antikoerper, similar to mKIAA0319 protein antikoerper, KIAA0319 antikoerper, D130043K22Rik antikoerper, RGD1307443 antikoerper

Hintergrund: Name/Gene ID: KIAA0319

Synonyms: KIAA0319, Dyslexia susceptibility 2, DYX2, DYLX2

Gen-ID: 9856

NCBI Accession: NP_001074520

UniProt: Q5VV43