RFP Antikörper (Red Fluorescent Protein)

Details for Product anti-RFP Antibody No. ABIN334653
Antigen
  • RFP
  • RNF76
  • tripartite motif containing 27
  • TRIM27
Reaktivität
Discosoma
68
11
9
4
1
1
Wirt
Ratte
43
32
14
4
1
Klonalität (Klon)
Monoklonal ()
Konjugat
Dieser RFP Antikörper ist unkonjugiert
3
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
Applikation
Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB)
67
36
25
22
18
14
6
4
2
1
1
1
1
1
1
1
Optionen
Verwendungszweck For biochemical analysis of RFP-tagged fusion proteins.
Klon 5F8
Isotyp IgG2a
Spezifität 5F8 efficiently recognizes monomeric red fluorescent proteins from Discosoma sp. derived from DsRed such as mRFP, mCherry or mPlum but no green fluorescent proteins.
Tested on mRFP, mCherry, mPlum, mOrange, mRFPRuby, tdTomato, DsRed
Sensitivität Sensitivity range of rat RFP antibody 5F8 goes down to 1.25 ng of purified RFP protein.
Produktmerkmale Use this rat monoclonal antibody (MAb) against monomeric RFPs, designated as RFP antibody [5F8]. The 5F8 MAb is characterized by high affinity and specificity against DsRed derivatives. It is suitable for ELISA, immunoblotting, immunoprecipitation, and immunofluorescence assays.
Reinigung Hybridoma supernatant. The Hybridoma was cultured in protein free medium. Tissue culture supernatant was concentrated by centrifugation using a 30 kDa exclusion membrane to an antibody concentration of 1 mg/ml.
Plasmids, Primers & others Plasmide, Primers & weitere RFP products on genomics-online (e.g. as negative or positive controls)
Antigen
Andere Bezeichnung RFP (RFP Antibody Abstract)
Hintergrund Fluorescent proteins (FP) are powerful tools to study protein localization and dynamics in living cells. In addition to the prominent green fluorescent protein (GFP) from Aequorea victoria, a red fluorescent protein (DsRed) was discovered in the coral Discosoma sp. Mutagenesis of the red progenitor have resulted in several monomeric red FPs (mRFP1, mCherry, mOrange, mPlum, etc.) which became attractive tools for a number of applications.
Applikations-hinweise Western blot: recommended starting concentration 1/1000
IF: recommended starting concentration 1/1000
IP: recommended starting volume 10 µL/IP
Beschränkungen Nur für Forschungszwecke einsetzbar
Format Liquid
Konzentration 1mg/mL
Konservierungs-mittel Sodium azide
Vorsichtsmaßnahmen This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handhabung Do not freeze.
Lagerung 4 °C
Haltbarkeit 12 months
Bilder des Herstellers
Image no. 1 for anti-Red Fluorescent Protein (RFP) antibody (ABIN334653) primary antibody: 0.2 ug of multi-red antibody 5F8 incubation for 1 h at RT secondary...
Image no. 2 for anti-Red Fluorescent Protein (RFP) antibody (ABIN334653) RFP antibody 5F8 shows no crossreactivity to any cellular protein. Detects a band of ...
Image no. 3 for anti-Red Fluorescent Protein (RFP) antibody (ABIN334653) Immunofluorescence: HeLa cells stably expressing mCherry-PCNA, 3.7% PFA fixed. Primar...
Image no. 4 for anti-Red Fluorescent Protein (RFP) antibody (ABIN334653) Sensitivity range of rat RFP antibody 5F8 goes down to1.25 ng of purified RFP protein.
Image no. 5 for anti-Red Fluorescent Protein (RFP) antibody (ABIN334653) IP, Co-IP: For Co-immunoprecipitation, 4 µg of RFP antibody 5F8 was used incubation f...
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