PRPF31 Antikörper (Internal Region)

Produktnummer ABIN334465

Produktdetails anti-PRPF31 Antikörper

Target: PRPF31 (Pre-mRNA Processing Factor 31 (PRPF31))

Bindungsspezifität: Internal Region

Reaktivität: Human

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser PRPF31 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)

Verwendungszweck: PRPF31

Sequenz: KELGNSLDKC KNNEN

Kreuzreaktivität: Rind (Kuh), Hund, Human, Maus, Schwein, Ratte

Aufreinigung: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Güteklasse: Verified

Immunogen: Peptide with sequence KELGNSLDKCKNNEN, from the internal region of the protein sequence according to NP_056444.3.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Western Blot: Approx 60 kDa band observed in nuclear lysates of cell lines A431, HeLa, HEK293 and NIH3T3 (calculated MW of 55.4 kDa according to Human NP_056444.3 and Mouse NP_081604.3). Recommended concentration: 1-3 μg/mL. Primary incubation 1 hour at roo

Peptide ELISA: antibody detection limit dilution 1:128000.

Kommentare:

Immunofluorescence: Strong expression of the protein seen in the nuclei and cytoplasm of HEK293 and HeLa cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of HEK293 cells. Recommended

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Details zu PRPF31

Target: PRPF31 (Pre-mRNA Processing Factor 31 (PRPF31))

Andere Bezeichnung: PRPF31 (PRPF31 Produkte)

Synonyme: DDBDRAFT_0219465 antikoerper, DDBDRAFT_0304959 antikoerper, DDB_0219465 antikoerper, DDB_0304959 antikoerper, PRPF31 antikoerper, NY-BR-99 antikoerper, PRP31 antikoerper, RP11 antikoerper, SNRNP61 antikoerper, prp31 antikoerper, rp11 antikoerper, 1500019O16Rik antikoerper, 2810404O06Rik antikoerper, AW554706 antikoerper, zgc:66177 antikoerper, pre-mRNA processing factor 31 antikoerper, PrpF31 U4/U6*U5 snRNP-associated pre-mRNA processing factor 31, (IC) antikoerper, U4/U6 small nuclear ribonucleoprotein Prp31 antikoerper, pre-mRNA processing factor 31 S homeolog antikoerper, PRP31 pre-mRNA processing factor 31 homolog (yeast) antikoerper, PRPF31 antikoerper, prpf31 antikoerper, PrpF31 antikoerper, LOC100455616 antikoerper, Prpf31 antikoerper, prpf31.S antikoerper

Hintergrund: PRPF31, PRP31 pre-mRNA processing factor 31 homolog (S. cerevisiae), DKFZp566J153, NY-BR-99, PRP31, RP11 , PRP31 pre-mRNA processing factor 31 homolog (yeast), pre-mRNA processing factor 31 homolog, pre-mRNA processing factor 31 homolog (yeast)

Gen-ID: 26121, 68988

NCBI Accession: NP_056444

Pathways: Ribonucleoprotein Complex Subunit Organization