TNF alpha Antikörper (AA 77-232)

Produktnummer ABIN3201320

Produktdetails anti-TNF alpha Antikörper

Target: TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Bindungsspezifität: AA 77-232

Reaktivität: Schwein

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser TNF alpha Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Verwendungszweck: Monoclonal Antibody to Tumor Necrosis Factor Alpha (TNFa)

Sequenz: MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- LRSS SQTSDKPVAH VVANVKAEGQ LQWQSGYANA LLANGVKLKD NQLVVPTDGL YLIYSQVLFR GQGCPSTNVF LTHTISRIAV SYQTKVNLLS AIKSPCQRET PEGAEAKPWY EPIYLGGVFQ LEKDDRLSAE INLPDYLDFA ESGQVYFGII AL

Spezifität: The antibody is a mouse monoclonal antibody raised against TNFa. It has been selected for its ability to recognize TNFa in immunohistochemical staining and western blotting.

Kreuzreaktivität: Human

Aufreinigung: Protein A + Protein G affinity chromatography

Immunogen: Recombinant Tumor Necrosis Factor Alpha (TNFa) corresdonding to Leu77~Leu232 with N-terminal His Tag

Klon: C9

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise:

Western blotting: 0.5-5 μg/mL Immunohistochemistry: 5-30 μg/mL Immunocytochemistry: 5-30 μg/mL Optimal working dilutions must be determined by end user.

Kommentare:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handhabung: Avoid repeated freeze/thaw cycles

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Haltbarkeit: 24 months

Details zu TNF alpha

Target: TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Andere Bezeichnung: Tumor Necrosis Factor Alpha (TNF alpha Produkte)

Synonyme: DIF antikoerper, TNF-alpha antikoerper, TNFA antikoerper, TNFSF2 antikoerper, RATTNF antikoerper, Tnfa antikoerper, tnf antikoerper, TNF-a antikoerper, TNFalpha antikoerper, Tnfsf1a antikoerper, TNFa antikoerper, cTNF antikoerper, Tnf-alpha antikoerper, tnfa-like antikoerper, TNF-ALPHA antikoerper, dif antikoerper, tnfa antikoerper, xtnf antikoerper, tnfsf2 antikoerper, tnf-alpha antikoerper, Cachectin antikoerper, tumor necrosis factor antikoerper, tumor necrosis factor b (TNF superfamily, member 2) antikoerper, tumor necrosis factor alpha antikoerper, tumor necrosis factor a (TNF superfamily, member 2) antikoerper, TNF antikoerper, Tnf antikoerper, tnf antikoerper, tnfb antikoerper, tnf-alpha antikoerper, LOC103694380 antikoerper, tnfa antikoerper

Hintergrund: DIF, TNF-A, TNFSF2, Cachectin, Tumor Necrosis Factor Ligand Superfamily Member 2

Pathways: NF-kappaB Signalweg, Apoptose, Caspase Kaskade in der Apoptose, TLR Signalweg, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Inflammasome