ELISA: 1: 40000approx. 1: 60000. WB: 1: 500approx. 1: 1000. IHC: 1: 50approx. 1: 200. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Several oligosaccharide structures and protein glycoconjugate types are found in nature. Homologous glycosyltransferase (GT) gene families catalyze the formation of glycosidic linkages. The β-1,3 galactosyltransferase(β3GalT) gene family encodes a set of type II transmembrane glycoproteins that are catalytically diverse and use different donor substrates (UDP-galactose and UDP-N-acetylglucosamine) and different acceptor sugars (N-acetylglucosamine, galactose, N-acetylgalactosamine ) to catalyze the addition of an activated monosaccharide to a terminal lactose. The protein coding sequences for β-1,3-Gal-T genes comprise a single exon and are distantly related to the Drosophila Brainiac gene. The β-1,4-galactosyltransferase (β4GalT) gene family encodes type II membrane-bound glycoproteins that show exclusive specificity for the donor substrate, UDP-galactose. β-1,4Gal-T genes transfer galactose in a β-1,4 linkage to similar acceptor sugars, each gene has a distinct function in the biosynthesis of different glycoconjugates and saccharide structures.Synonyms: 4-GalTase 3, 4-galactosyltransferase 3, Beta-1, Beta-1, Beta4Gal-T3, UDP-Gal:beta-GlcNAc beta-1, UDP-galactose:beta-N-acetylglucosamine beta-1, b4Gal-T3