BRF1 Antikörper

Produktnummer ABIN317615

Produktdetails anti-BRF1 Antikörper

Target: BRF1 (BRF1, RNA polymerase III transcription initiation factor subunit (BRF1))

Reaktivität: Human, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser BRF1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Spezifität: This antibody detects endogenous levels of TFIIIB90-1 protein.(region surrounding Glu257)

Aufreinigung: Affinity Chromatography using epitope-specific immunogen

Anwendungsinformationen

Applikationshinweise: Western Blot: 1/500-1/1000. ELISA: 1/1000-1/5000. Immunohistochemistry: 1/50-1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH ~7.2, 0.05 % Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Details zu BRF1

Target: BRF1 (BRF1, RNA polymerase III transcription initiation factor subunit (BRF1))

Andere Bezeichnung: BRF1 / TFIIIB90 (BRF1 Produkte)

Synonyme: BRF antikoerper, BRF-1 antikoerper, GTF3B antikoerper, TAF3B2 antikoerper, TAF3C antikoerper, TAFIII90 antikoerper, TF3B90 antikoerper, TFIIIB90 antikoerper, hBRF antikoerper, DDBDRAFT_0189159 antikoerper, DDBDRAFT_0216283 antikoerper, DDB_0189159 antikoerper, DDB_0216283 antikoerper, 2510002F24Rik antikoerper, mTFIIIB90 antikoerper, BRF1 antikoerper, ZFP36L1 antikoerper, CG31256 antikoerper, CG4155 antikoerper, CG5419 antikoerper, Dmel\\CG31256 antikoerper, TFIIIB antikoerper, brf antikoerper, brf1 antikoerper, wu:fi47f03 antikoerper, zgc:55543 antikoerper, fi40c10 antikoerper, wu:fi40c10 antikoerper, zgc:63497 antikoerper, BRF1, RNA polymerase III transcription initiation factor subunit antikoerper, transcription initiation factor IIB antikoerper, general transcription factor IIB antikoerper, cilia and flagella associated protein 20 antikoerper, transcription factor IIb, putative antikoerper, transcription factor IIB antikoerper, transcription factor IIb antikoerper, Transcription factor iib, putative antikoerper, BRF1, RNA polymerase III transcription initiation factor 90 kDa subunit antikoerper, CG31256 gene product from transcript CG31256-RA antikoerper, BRF1, RNA polymerase III transcription initiation factor a antikoerper, BRF1, RNA polymerase III transcription initiation factor b antikoerper, BRF1, RNA polymerase III transcription initiation factor 90 kDa subunit L homeolog antikoerper, BRF1 antikoerper, gtf2b antikoerper, GTF2B antikoerper, cfap20 antikoerper, PFE0415w antikoerper, Tb09.160.4220 antikoerper, PVX_097980 antikoerper, CpipJ_CPIJ011738 antikoerper, CpipJ_CPIJ014799 antikoerper, CpipJ_CPIJ018710 antikoerper, PKH_102450 antikoerper, Brf1 antikoerper, Brf antikoerper, brf1a antikoerper, brf1b antikoerper, brf1.L antikoerper

Hintergrund: RNA polymerase (pol) III synthesizes tRNA, 5s rRNA, 7SL RNA and U6 snRNA and is overexpressed in many transformed cell lines and tumors in vivo, since cells must duplicate its protein components before division. Therefore, in order to maintain rapid growth, cells must produce a high level of Pol III transcribed RNA, which requires the presence of the TFIIIB and TFIIIC2 transcription factor complexes. The TFIIIC2 complex is composed of five subunits, TFIIIC220, TFIIIC110, TFIIIC102, TFIIIC90 and TFIIIC63, that are overexpressed in adenovirus transformed cells as well as in alignant cells in vivo, such as ovarian carcinomas. TFIIIC2 recruits RNA pol III and TFIIIB to promoter elements and may be a key component in the deregulation of malignant cells. The TFIIIB complex includes the TATA-binding protein (TBP), TFIIB-related factor 1 (TFIIIB90, BRF1) and TFIIIB, the expression of which are also upregulated in transformed cells. In many carcinomas, the tumor suppressors retinoblastoma (RB) and p53 are inactivated, which affects their ability to bind and inactivate the function of TFIIIB.Synonyms: B-related factor 1, BRF, GTF3B, RNA polymerase III subunit 2, TAF3B2, TAF3C, TATA box-binding protein-associated factor, Transcription factor IIIB 90 kDa subunit

Molekulargewicht: approx. 74 kDa

Gen-ID: 2972

NCBI Accession: NP_001510

UniProt: Q92994