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TNF alpha Antikörper

TNF alpha Reaktivität: Human, Maus, Affe, Meerschweinchen WB, ELISA, IHC, FACS, IHC (p), IHC (fro) Wirt: Maus Monoclonal 52B83 unconjugated
Produktnummer ABIN305730
  • Target Alle TNF alpha Antikörper anzeigen
    TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))
    Reaktivität
    • 333
    • 202
    • 129
    • 67
    • 51
    • 42
    • 27
    • 25
    • 23
    • 16
    • 11
    • 10
    • 10
    • 9
    • 7
    • 5
    • 5
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    Human, Maus, Affe, Meerschweinchen
    Wirt
    • 262
    • 203
    • 46
    • 26
    • 6
    • 3
    • 2
    • 2
    • 2
    Maus
    Klonalität
    • 279
    • 264
    Monoklonal
    Konjugat
    • 260
    • 89
    • 45
    • 20
    • 19
    • 13
    • 8
    • 8
    • 6
    • 6
    • 6
    • 6
    • 6
    • 6
    • 6
    • 5
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Dieser TNF alpha Antikörper ist unkonjugiert
    Applikation
    • 354
    • 217
    • 139
    • 130
    • 74
    • 64
    • 57
    • 54
    • 49
    • 42
    • 39
    • 38
    • 37
    • 32
    • 20
    • 12
    • 10
    • 9
    • 9
    • 6
    • 6
    • 6
    • 5
    • 5
    • 4
    • 4
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))
    Marke
    IHC-plus™
    Spezifität
    Recognizes human native and recombinant TNF-alpha. Reacts with soluble (17kD) and membrane (26kD) human TNF-alpha. Does not cross-react with TNF-beta, lymphotoxin and receptor bound TNF-alpha.
    Aufreinigung
    Purified
    Immunogen
    Human TNFa.
    Klon
    52B83
    Isotyp
    IgG1
    Top Product
    Discover our top product TNF alpha Primärantikörper
  • Applikationshinweise
    Approved: ELISA, Flo, IHC, IHC-Fr, IHC-P (2.5 μg/mL), WB (1:5)

    Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 2.5 μg/mL. Non-specific background staining is observed in connective tissues.
    Kommentare

    Target Species of Antibody: Human

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Liquid
    Konzentration
    Lot specific
    Buffer
    PBS, 0.02 % sodium azide, 0.1 % BSA.
    Konservierungsmittel
    Sodium azide
    Vorsichtsmaßnahmen
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handhabung
    Avoid repeat freeze-thaw cycles.
    Lagerung
    4 °C,-20 °C
    Informationen zur Lagerung
    Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
  • Target
    TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))
    Andere Bezeichnung
    TNF Alpha (TNF alpha Produkte)
    Synonyme
    DIF antikoerper, TNF-alpha antikoerper, TNFA antikoerper, TNFSF2 antikoerper, RATTNF antikoerper, Tnfa antikoerper, tnf antikoerper, TNF-a antikoerper, TNFalpha antikoerper, Tnfsf1a antikoerper, TNFa antikoerper, cTNF antikoerper, Tnf-alpha antikoerper, tnfa-like antikoerper, TNF-ALPHA antikoerper, dif antikoerper, tnfa antikoerper, xtnf antikoerper, tnfsf2 antikoerper, tnf-alpha antikoerper, Cachectin antikoerper, tumor necrosis factor antikoerper, tumor necrosis factor b (TNF superfamily, member 2) antikoerper, tumor necrosis factor alpha antikoerper, tumor necrosis factor a (TNF superfamily, member 2) antikoerper, TNF antikoerper, Tnf antikoerper, tnf antikoerper, tnfb antikoerper, tnf-alpha antikoerper, LOC103694380 antikoerper, tnfa antikoerper
    Hintergrund
    Name/Gene ID: TNF
    Family: TNF

    Synonyms: TNF, APC1 protein, Cachectin, DIF, TNF Alpha, Tumor necrosis factor-alpha, TNF-alpha, TNF-a, TNFA, Tumor necrosis factor, TNF, macrophage-derived, TNF, monocyte-derived, TNFSF2
    Gen-ID
    7124
    UniProt
    P01375
    Pathways
    NF-kappaB Signalweg, Apoptose, Caspase Kaskade in der Apoptose, TLR Signalweg, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Inflammasome
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