SNAP25 Antikörper (C-Term)

Produktnummer ABIN3044363

Produktdetails anti-SNAP25 Antikörper

Target: SNAP25 (Synaptosomal-Associated Protein, 25kDa (SNAP25))

Bindungsspezifität: AA 186-200, C-Term

Reaktivität: Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser SNAP25 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Verwendungszweck: Rabbit IgG polyclonal antibody for Synaptosomal-associated protein 25(SNAP25) detection. Tested with WB, IHC-P in Human,Mouse,Rat.

Sequenz: DSNKTRIDEA NQRAT

Kreuzreaktivität (Details): Predicted Cross Reactivity: human, mouse
No cross reactivity with other proteins.
Predicted Cross Reactivity: Species predicted to be fit for the product based on sequence similarities.

Produktmerkmale: Rabbit IgG polyclonal antibody for Synaptosomal-associated protein 25(SNAP25) detection. Tested with WB, IHC-P in Human,Mouse,Rat.
Gene Name: synaptosomal-associated protein, 25 kDa
Protein Name: Synaptosomal-associated protein 25(SNAP-25)

Aufreinigung: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human SNAP25(186-200aa DSNKTRIDEANQRAT), identical to the related rat and mouse sequences.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Rat, Predicted Species: Human, Mouse
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Rat, Predicted Species: Human, Mouse, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.

Kommentare:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Konservierungsmittel: Thimerosal (Merthiolate), Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Haltbarkeit: 12 months

Referenzen für anti-SNAP25 Antikörper (ABIN3044363)

Zhong, Xiang, Hu, Wang, Zeng, Wang, Xia, Wang, Zhang: "Synaptosomal-associated protein 25 may be an intervention target for improving sensory and locomotor functions after spinal cord contusion." in: Neural regeneration research, Vol. 12, Issue 6, pp. 969-976, (2017) (PubMed).

Details zu SNAP25

Target: SNAP25 (Synaptosomal-Associated Protein, 25kDa (SNAP25))

Andere Bezeichnung: SNAP25 (SNAP25 Produkte)

Synonyme: RIC-4 antikoerper, RIC4 antikoerper, SEC9 antikoerper, SNAP antikoerper, SNAP-25 antikoerper, bA416N4.2 antikoerper, dJ1068F16.2 antikoerper, SNAP-25B antikoerper, SNAP-25a antikoerper, ric-4 antikoerper, ric4 antikoerper, sec9 antikoerper, snap-25 antikoerper, Bdr antikoerper, sp antikoerper, fj55h11 antikoerper, snap25 antikoerper, wu:fj55h11 antikoerper, Snap antikoerper, snap25.2 antikoerper, wu:fj33h05 antikoerper, synaptosome associated protein 25 antikoerper, synaptosome associated protein 25kDa S homeolog antikoerper, synaptosomal-associated protein, 25kDa antikoerper, synaptosomal-associated protein 25 antikoerper, synaptosome associated protein 25kDa antikoerper, synaptosome associated protein 25kDa L homeolog antikoerper, zgc:101731 antikoerper, synaptosomal-associated protein, 25b antikoerper, SNAP25 antikoerper, Snap25 antikoerper, snap25.S antikoerper, snap25 antikoerper, BACOIKO003_07 antikoerper, Tsp_10811 antikoerper, snap25.L antikoerper, zgc:101731 antikoerper, snap25b antikoerper

Hintergrund: Synaptosome-associated protein of 25,000 daltons also known as SNAP-25 is a protein which in humans encodes a 25-kD protein of 206 amino acids. It was first investigated as a neuron-specific gene preferentially expressed in mouse hippocampus. The tSNARE(the target-membrane soluble NSF-attachment protein receptor, where NSF is N-ethylmaleimide-sensitive fusion protein) synaptosomal-associated protein of 25 kDa(SNAP-25) is expressed in pancreatic B-cells and its cleavage by botulinum neurotoxin E(BoNT/E) abolishes stimulated secretion of insulin. In the nervous system, two SNAP-25 isoforms(a and b) have been described, which are produced by alternative splicing. Nagy et al.(2004) identified mammalian Snap25a and Snap25b as targets of protein kinase A, a key regulator of neurosecretion that primes slowly releasable pools and readily releasable pools of secretory vesicles. SNAP-25 inhibits P/Q- and L-type voltage-gated calcium channels located presynaptically and interacts with the synaptotagmin C2B domain in Ca2+-independent fashion. In glutamatergic synapses SNAP-25 decreases the Ca2+ responsiveness, while it is naturally absent in GABAergic synapses.

Synonyms: bA416N4.2 antibody|Bdr antibody|dJ1068F16.2 antibody|FLJ23079 antibody|HGNC:11132 antibody|MGC105414 antibody|MGC139754 antibody|Resistance to inhibitors of cholinesterase 4 homolog antibody|RIC 4 antibody|RIC4 antibody|SEC 9 antibody|SEC9 antibody|SNAP 25 antibody|SNAP antibody|SNAP-25 antibody|SNAP-25B antibody|SNAP25 antibody|SNP 25 antibody|SNP25 antibody|SNP25_HUMAN antibody|sp antibody|SUP antibody|Super protein antibody|Synaptosomal associated 25 kDa protein antibody|Synaptosomal associated protein antibody|Synaptosomal Associated Protein 25 antibody|Synaptosomal associated protein 25 kDa antibody|Synaptosomal-associated 25 kDa protein antibody|Synaptosomal-associated protein 25 antibody|Synaptosomal-associated protein, 25-KD antibody

UniProt: P60880

Pathways: Positive Regulation of Peptide Hormone Secretion, Hormone Transport, Synaptic Vesicle Exocytosis, Dicarboxylic Acid Transport