CXCR4 Antikörper (N-Term)

Produktnummer ABIN3044349

Produktdetails anti-CXCR4 Antikörper

Target: CXCR4 (Chemokine (C-X-C Motif) Receptor 4 (CXCR4))

Bindungsspezifität: AA 18-33, N-Term

Reaktivität: Human, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser CXCR4 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Verwendungszweck: Rabbit IgG polyclonal antibody for C-X-C chemokine receptor type 4(CXCR4) detection. Tested with WB, IHC-P in Human,Mouse,Rat.

Sequenz: SGDYDSMKEP CFREEN

Kreuzreaktivität (Details): Predicted Cross Reactivity: mouse
No cross reactivity with other proteins.
Predicted Cross Reactivity: Species predicted to be fit for the product based on sequence similarities.

Produktmerkmale: Rabbit IgG polyclonal antibody for C-X-C chemokine receptor type 4(CXCR4) detection. Tested with WB, IHC-P in Human,Mouse,Rat.
Gene Name: chemokine(C-X-C motif) receptor 4
Protein Name: C-X-C chemokine receptor type 4(CXC-R4/CXCR-4)

Aufreinigung: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human CXCR4(18-33aa SGDYDSMKEPCFREEN), different from the related rat and mouse sequences by two amino acids.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Rat, Predicted Species: Mouse
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.

Kommentare:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Konservierungsmittel: Thimerosal (Merthiolate), Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Referenzen für anti-CXCR4 Antikörper (ABIN3044349)

Zhang, Wang, Yue, Liu, Liu: "Construction of a CXCL12-KDEL fusion gene to inhibit head and neck squamous cell carcinoma metastasis by intracellular sequestration of CXCR4." in: BioMed research international, Vol. 2015, pp. 195828, (2015) (PubMed).

Zhu, Sun, Tan, Xu, Dai, Wang, Fan, Zhou: "Tacrolimus promotes hepatocellular carcinoma and enhances CXCR4/SDF‑1α expression in vivo." in: Molecular medicine reports, Vol. 10, Issue 2, pp. 585-92, (2015) (PubMed).

Liu, Wang, Wang, Wang: "Anticancer effects of chemokine receptor 4(CXCR4) gene silenced by CXCR4-siRNA in nude mice model of ovarian cancer." in: Cell biochemistry and biophysics, Vol. 70, Issue 3, pp. 1893-900, (2014) (PubMed).

Li, Chen, Yuan, Zhou, He, Zu, Qi: "CXCR4 expression in bladder transitional cell carcinoma and its relationship with clinicopathological features." in: Urologia internationalis, Vol. 92, Issue 2, pp. 157-63, (2014) (PubMed).

Tan, Chang, Liu, Tang: "Silencing of CXCR4 inhibits tumor cell proliferation and neural invasion in human hilar cholangiocarcinoma." in: Gut and liver, Vol. 8, Issue 2, pp. 196-204, (2014) (PubMed).

Wang, Guo, Han, Xu, Guo, Sun: "Distinct roles of different fragments of PDCD4 in regulating the metastatic behavior of B16 melanoma cells." in: International journal of oncology, Vol. 42, Issue 5, pp. 1725-33, (2013) (PubMed).

Chen, Wang, Wang, Zhang, Liu, Pan, Wang, Chen, Jin, Wang: "Effect of short hairpin RNA-induced CXCR4 silence on ovarian cancer cell." in: Biomedicine & pharmacotherapy = Biome?decine & pharmacothe?rapie, Vol. 66, Issue 7, pp. 549-53, (2012) (PubMed).

Lin, Zheng, Shen, Tang, Chen, Sun, Zhao, Yao: "Relationships between levels of CXCR4 and VEGF and blood-borne metastasis and survival in patients with osteosarcoma." in: Medical oncology (Northwood, London, England), Vol. 28, Issue 2, pp. 649-53, (2011) (PubMed).

Wang, Cai, Han, Yang, Tong, Cao, Wu, Wang: "Silencing of CXCR4 blocks progression of ovarian cancer and depresses canonical Wnt signaling pathway." in: International journal of gynecological cancer : official journal of the International Gynecological Cancer Society, Vol. 21, Issue 6, pp. 981-7, (2011) (PubMed).

Li, Deng, Gong, Bian, Dai, Wang: "Upregulation of CXCR4 favoring neural-like cells migration via AKT activation." in: Neuroscience research, Vol. 67, Issue 4, pp. 293-9, (2010) (PubMed).

Zhang, Qi, Li, Zhang, Xu, Wang, Sun: "Chemokine CXCL12 and its receptor CXCR4 expression are associated with perineural invasion of prostate cancer." in: Journal of experimental & clinical cancer research : CR, Vol. 27, pp. 62, (2009) (PubMed).

Kotsev, Pavlova, Zhecheva, Gencheva: "[Blue sclera in iron-deficiency states]." in: Vŭtreshni bolesti, Vol. 28, Issue 4, pp. 145-7, (1990) (PubMed).

Details zu CXCR4

Target: CXCR4 (Chemokine (C-X-C Motif) Receptor 4 (CXCR4))

Andere Bezeichnung: CXCR4 (CXCR4 Produkte)

Synonyme: CD184 antikoerper, D2S201E antikoerper, FB22 antikoerper, HM89 antikoerper, HSY3RR antikoerper, LAP3 antikoerper, LCR1 antikoerper, LESTR antikoerper, NPY3R antikoerper, NPYR antikoerper, NPYRL antikoerper, NPYY3R antikoerper, WHIM antikoerper, Cmkar4 antikoerper, PB-CKR antikoerper, PBSF/SDF-1 antikoerper, Sdf1r antikoerper, CXC-R4-B antikoerper, CXCR-4-B antikoerper, cd184 antikoerper, cxcr4 antikoerper, fb22 antikoerper, hm89 antikoerper, hsy3rr antikoerper, lap3 antikoerper, lcr1 antikoerper, lestr antikoerper, npy3r antikoerper, npyr antikoerper, npyrl antikoerper, npyy3r antikoerper, xcxcr4 antikoerper, CXC-R4 antikoerper, CXCR-4 antikoerper, d2s201e antikoerper, whim antikoerper, CXC-R4-A antikoerper, CXCR-4-A antikoerper, xCXCR4 antikoerper, CXCR4 antikoerper, cb403 antikoerper, zgc:109863 antikoerper, cb824 antikoerper, C-X-C motif chemokine receptor 4 antikoerper, chemokine (C-X-C motif) receptor 4 antikoerper, C-X-C motif chemokine receptor 4 S homeolog antikoerper, C-X-C chemokine receptor type 4 antikoerper, C-X-C motif chemokine receptor 4 L homeolog antikoerper, chemokine (C-X-C motif), receptor 4b antikoerper, chemokine (C-X-C motif) receptor 4a antikoerper, CXCR4 antikoerper, Cxcr4 antikoerper, cxcr4.S antikoerper, cxcr4 antikoerper, LOC100049444 antikoerper, cxcr4.L antikoerper, cxcr4b antikoerper, cxcr4a antikoerper

Hintergrund: CXCR4 (Chemokine,CXC Motif, Receptor 4), also known as FUSIN or NPY3R, is a protein that in humans is encoded by the CXCR4 gene. It is the receptor for the CXC chemokine SDF1 that has essential functions on embryo organogenesis, immunological functions and T lymphocyte trafficking. CXCR4 is the only SDF1 receptor identified so far. This suggests that CXCR4 expression is critical for the biological effects of SDF1. CXCR4 is also a seven-transmembrane-spanning, G-protein-coupled receptor for the CXC chemokine PBSF/SDF-1. It functions as a co-receptor for T-cell-line tropic human immunodeficiency virus HIV-1. It was concluded that PBSF/SDF-1 and CXCR4 define a new signalling system for organ vascularization.

Synonyms: C-X-C chemokine receptor type 4 antibody|CD184 antibody|CD184 antigen antibody|Chemokine(C X C motif) receptor 4 antibody|Chemokine CXC Motif Receptor 4 antibody|CXC-R4 antibody|CXCR-4 antibody|CXCR4 antibody|CXCR4_HUMAN antibody|D2S201E antibody|FB22 antibody|Fusin antibody|HM89 antibody|HSY3RR antibody|LAP 3 antibody|LAP3 antibody|LCR1 antibody|LESTR antibody|Leukocyte derived seven transmembrane domain receptor antibody|Leukocyte-derived seven transmembrane domain receptor antibody|Lipopolysaccharide associated protein 3 antibody|Neuropeptide Y receptor Y3 antibody|NPY3R antibody|NPYR antibody|NPYRL antibody|NPYY3 antibody|NPYY3R antibody|Probable G protein coupled receptor lcr1 homolog antibody|SDF 1 receptor antibody|SDF-1 receptor antibody|SEVEN-TRANSMEMBRANE-SEGMENT RECEPTOR antibody|Stromal cell derived factor 1 receptor antibody|Stromal cell-derived factor 1 receptor antibody|WHIM antibody

UniProt: P61073

Pathways: Regulation of Cell Size, CXCR4-mediated Signaling Events