SHC1 Antikörper (C-Term)

Produktnummer ABIN3044111

Produktdetails anti-SHC1 Antikörper

Target: SHC1 (SHC (Src Homology 2 Domain Containing) Transforming Protein 1 (SHC1))

Bindungsspezifität: AA 424-440, C-Term

Reaktivität: Human, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser SHC1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro))

Verwendungszweck: Rabbit IgG polyclonal antibody for SHC-transforming protein 1(SHC1) detection. Tested with WB, IHC-P, IHC-F, ICC in Human,Mouse,Rat.

Sequenz: DPSYVNVQNL DKARQAV

Kreuzreaktivität (Details): Predicted Cross Reactivity: mouse
No cross reactivity with other proteins.
Predicted Cross Reactivity: Species predicted to be fit for the product based on sequence similarities.

Produktmerkmale: Rabbit IgG polyclonal antibody for SHC-transforming protein 1(SHC1) detection. Tested with WB, IHC-P, IHC-F, ICC in Human,Mouse,Rat.
Gene Name: SHC(Src homology 2 domain containing) transforming protein 1
Protein Name: SHC-transforming protein 1

Aufreinigung: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human SHC(424-440aa DPSYVNVQNLDKARQAV), different from the related rat and mouse sequences by two amino acids.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Rat, Predicted Species: Mouse, The detection limit for SHC1 is approximately 1 ng/lane under reducing conditions.
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Rat, Predicted Species: Mouse, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
IHC-F: Concentration: 0.5-1 μg/mL, Tested Species: Rat, Predicted Species: Human, Mouse
ICC: Concentration: 0.5-1 μg/mL, Tested Species: Human
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.

Kommentare:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P), IHC(F) and ICC.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Konservierungsmittel: Thimerosal (Merthiolate), Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Haltbarkeit: 12 months

Details zu SHC1

Target: SHC1 (SHC (Src Homology 2 Domain Containing) Transforming Protein 1 (SHC1))

Andere Bezeichnung: SHC1 (SHC1 Produkte)

Synonyme: SHC1 antikoerper, SHC antikoerper, SHCA antikoerper, P66shc antikoerper, si:dkey-246i14.4 antikoerper, wu:fc10h03 antikoerper, shc antikoerper, p66shc antikoerper, Shc antikoerper, ShcA antikoerper, p66 antikoerper, SHC adaptor protein 1 antikoerper, Shc1p antikoerper, squalene--hopene cyclase antikoerper, SHC (Src homology 2 domain containing) transforming protein 1 antikoerper, SHC (Src homology 2 domain containing) transforming protein 1 L homeolog antikoerper, src homology 2 domain-containing transforming protein C1 antikoerper, SHC1 antikoerper, shc1 antikoerper, Shc1 antikoerper, shc1.L antikoerper

Hintergrund: SHC1(SHC-transforming protein 1), also known as SHCA, is a protein that in humans is encoded by the SHC1 gene. SCOP classifies the 3D structure as belonging to the SH2 domain family. By Southern analysis of somatic cell hybrids followed by both isotopic and fluorescence in situ hybridization, Huebner et al.(1994) assigned the SHC1 gene to 1q21. Yulug et al.(1995) used fluorescence in situ hybridization to map the SHC1 gene to 1q21. By the same method, an SHC-related sequence(SHCL1) was mapped to 17q21-q22. By FISH analysis and direct sequencing of vectorette library PCR products, Harun et al.(1997) identified SHC1P1, a 3.2-kb processed pseudogene, in Xq12-q13.1. SHC1P1 is 85 % identical to mouse Shc p66. Reporter assays showed FKHRL1 transactivates CAT, suggesting a capacity to augment antioxidant scavenging. Nemoto and Finkel(2002) concluded that there is an important functional relationship between forkhead proteins(e.g., FKHRL1), SHC1, and intracellular oxidants, all of which are though to be involved in the aging process in worms and mammals.

Synonyms: FLJ26504 antibody|MGC98812 antibody|p46 antibody|p52 antibody|p52 SHC antibody|p52SHC antibody|p66 antibody|p66 SHC antibody|p66SHC antibody|SH2 domain protein C1 antibody|SHC(Src homology 2 domain containing) transforming protein 1 antibody|SHC 1 antibody|SHC A antibody|Shc antibody|SHC transforming protein 1 antibody|SHC transforming protein antibody|SHC-transforming protein 1 antibody|SHC-transforming protein 3 antibody|SHC-transforming protein A antibody|SHC1 antibody|SHC1_HUMAN antibody|SHCA antibody|Src homology 2 domain containing transforming protein C1 antibody|Src homology 2 domain-containing-transforming protein C1 antibody

UniProt: P29353

Pathways: RTK Signalweg, T-Zell Rezeptor Signalweg, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung, ER-Nucleus Signaling, Signaling Events mediated by VEGFR1 and VEGFR2