PCSK1 Antikörper (C-Term)

Produktnummer ABIN3044077

Produktdetails anti-PCSK1 Antikörper

Target: PCSK1 (Proprotein Convertase Subtilisin/kexin Type 1 (PCSK1))

Bindungsspezifität: AA 726-740, C-Term

Reaktivität: Ratte, Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser PCSK1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Verwendungszweck: Rabbit IgG polyclonal antibody for Neuroendocrine convertase 1(PCSK1) detection. Tested with WB, IHC-P in Human,Mouse,Rat.

Sequenz: DVFYNTKPYK HRDDR

Kreuzreaktivität (Details): Predicted Cross Reactivity: mouse
No cross reactivity with other proteins.
Predicted Cross Reactivity: Species predicted to be fit for the product based on sequence similarities.

Produktmerkmale: Rabbit IgG polyclonal antibody for Neuroendocrine convertase 1(PCSK1) detection. Tested with WB, IHC-P in Human,Mouse,Rat.
Gene Name: proprotein convertase subtilisin/kexin type 1
Protein Name: Neuroendocrine convertase 1

Aufreinigung: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human PC1/3(726-740aa DVFYNTKPYKHRDDR), identical to the related mouse and rat sequences.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Rat, Predicted Species: Mouse, The detection limit for PCSK1 is approximately 1 ng/lane under reducing conditions.
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Rat, Predicted Species: Human, Mouse, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.

Kommentare:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Konservierungsmittel: Thimerosal (Merthiolate), Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Haltbarkeit: 12 months

Details zu PCSK1

Target: PCSK1 (Proprotein Convertase Subtilisin/kexin Type 1 (PCSK1))

Andere Bezeichnung: PCSK1 (PCSK1 Produkte)

Synonyme: BMIQ12 antikoerper, NEC1 antikoerper, PC1 antikoerper, PC3 antikoerper, SPC3 antikoerper, Nec-1 antikoerper, Nec1 antikoerper, Phpp-1 antikoerper, BDP antikoerper, PC1/3 antikoerper, proprotein convertase subtilisin/kexin type 1 antikoerper, prohormone convertase 1 antikoerper, PCSK1 antikoerper, Pcsk1 antikoerper, pcsk1 antikoerper, PC1B antikoerper

Hintergrund: PCSK1(Proprotein Convertase, Subtilisin/Kexin-Type, 1), also known as PC1 or NEC1, is an enzyme that in humans is encoded by the PCSK1 gene. Proprotein convertase-1 is a neuroendocrine convertase that belongs to a family of subtilisin-like serine endoproteases that process large precursor proteins into mature bioactive products. By in situ hybridization, Seidah et al.(1991) mapped the NEC1 gene to human chromosome 5q15-q21 and mouse chromosome 13. Ohagi et al.(1996) noted that PC1 initiates the sequential processing of proinsulin to insulin by cleaving the proinsulin molecule on the C-terminal side of the dibasic peptide, arg31-arg32, joining the B-chain and C-peptide. By observing the phenotypic features in patients with PC1 mutations, Jackson et al.(2003) concluded that human intestinal absorptive function is dependent on PC1 activity.

Synonyms: BDP antibody|EC antibody|NEC 1 antibody|NEC1 antibody|NEC1_HUMAN antibody|Neuroendocrine convertase 1 antibody|PC1 antibody|PC3 antibody|PCSK1 antibody|Prohormone convertase 1 antibody|Prohormone convertase 1/3 antibody|Prohormone convertase 3 antibody|Proprotein convertase 1 antibody|SPC3 antibody

UniProt: P29120

Pathways: Peptide Hormone Metabolism