SMAD5 Antikörper (Middle Region)

Produktnummer ABIN3043980

Produktdetails anti-SMAD5 Antikörper

Target: SMAD5 (SMAD, Mothers Against DPP Homolog 5 (SMAD5))

Bindungsspezifität: AA 248-267, Middle Region

Reaktivität: Human, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser SMAD5 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Verwendungszweck: Rabbit IgG polyclonal antibody for Mothers against decapentaplegic homolog 5(SMAD5) detection. Tested with WB, IHC-P in Human,Mouse,Rat.

Sequenz: IPQIMPSISS RDVQPVAYEE

Kreuzreaktivität (Details): Predicted Cross Reactivity: mouse
No cross reactivity with other proteins.
Predicted Cross Reactivity: Species predicted to be fit for the product based on sequence similarities.

Produktmerkmale: Rabbit IgG polyclonal antibody for Mothers against decapentaplegic homolog 5(SMAD5) detection. Tested with WB, IHC-P in Human,Mouse,Rat.
Gene Name: SMAD family member 5
Protein Name: Mothers against decapentaplegic homolog 5

Aufreinigung: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence in the middle region of human SMAD5(248-267aa IPQIMPSISSRDVQPVAYEE), identical to the related rat sequence, and different from the related mouse sequence by one amino acid.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Rat, Predicted Species: Mouse, The detection limit for SMAD5 is approximately 1.5 ng/lane under reducing conditions.
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Rat, Predicted Species: Mouse, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.

Kommentare:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Konservierungsmittel: Thimerosal (Merthiolate), Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Haltbarkeit: 12 months

Details zu SMAD5

Target: SMAD5 (SMAD, Mothers Against DPP Homolog 5 (SMAD5))

Andere Bezeichnung: SMAD5 (SMAD5 Produkte)

Synonyme: DWFC antikoerper, JV5-1 antikoerper, MADH5 antikoerper, Madh5 antikoerper, SBN antikoerper, fb67b04 antikoerper, mad antikoerper, madh5 antikoerper, sb:cb400 antikoerper, smd5 antikoerper, wu:fb67b04 antikoerper, SMAD5 antikoerper, DKFZp468M0619 antikoerper, 1110051M15Rik antikoerper, AI451355 antikoerper, Dwf-C antikoerper, MusMLP antikoerper, SMAD family member 5 antikoerper, SMAD5 antikoerper, Smad5 antikoerper, smad5 antikoerper

Hintergrund: Mother against decapentaplegic homolog 5 also known as SMAD5 is a protein that in humans is encoded by the SAMD5 gene. It belongs to the SMAD family of proteins, which belong to the TGFbeta superfamily of modulators. The gene was assigned to human chromosome 5q31. Like many other TGFbeta family members SMAD5 is involved in cell signalling and modulates signals of bone morphogenetic proteins(BMP's). It may play a role in the pathway where TGFbeta is an inhibitor of hematopoietic progenitor cells.

Synonyms: DKFZp781C1895 antibody|DKFZp781O1323 antibody|Dwfc antibody|hSmad 5 antibody|hSmad5 antibody|JV5 1 antibody|JV5-1 antibody|MAD homolog 5 antibody|MAD mothers against decapentaplegic homolog 5 antibody|MAD, mothers against decapentaplegic homolog 5 antibody|MADH 5 antibody|MADH5 antibody|Mothers against decapentaplegic homolog 5 antibody|Mothers against DPP homolog 5 antibody|MusMLP antibody|SMA and MAD related protein 5 antibody|SMAD 5 antibody|SMAD family member 5 antibody|SMAD mothers against DPP homolog 5 antibody|Smad5 antibody|SMAD5_HUMAN antibody

UniProt: Q99717