ENO2/NSE Antikörper (C-Term)

Produktnummer ABIN3043022

Produktdetails anti-ENO2/NSE Antikörper

Target: ENO2/NSE (ENO2) (Enolase 2 (Gamma, Neuronal) (ENO2))

Bindungsspezifität: AA 420-434, C-Term

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser ENO2/NSE Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Verwendungszweck: Rabbit IgG polyclonal antibody for Gamma-enolase(ENO2) detection. Tested with WB, IHC-P in Human,Mouse,Rat.

Sequenz: EARFAGHNFR NPSVL

Kreuzreaktivität (Details): Predicted Cross Reactivity: human, mouse
No cross reactivity with other proteins.
Predicted Cross Reactivity: Species predicted to be fit for the product based on sequence similarities.

Produktmerkmale: Rabbit IgG polyclonal antibody for Gamma-enolase(ENO2) detection. Tested with WB, IHC-P in Human,Mouse,Rat.
Gene Name: enolase 2(gamma, neuronal)
Protein Name: Gamma-enolase

Aufreinigung: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human NSE(420-434aa EARFAGHNFRNPSVL), identical to the related mouse and rat sequences.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Rat, Predicted Species: Human, Mouse
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Rat, Predicted Species: Human, Mouse, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.

Kommentare:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Konservierungsmittel: Thimerosal (Merthiolate), Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Referenzen für anti-ENO2/NSE Antikörper (ABIN3043022)

Zhai, Zhao, Zhou, Lu, Zhang, Na, Feng, Qiang, Du: "The association and significance of H3K27me3 and a folate metabolic gene ACat2 in neural tube defects." in: Nutrition journal, Vol. 15, Issue 1, pp. 95, (2017) (PubMed).

Xu, Lu, Miao, Wu, -Z Jiang, Ge, Fang, Zhu, Chen, Zhou, Lu, Tang, Wang: "Immunoregulatory effect of neuronal-like cells in inducting differentiation of bone marrow mesenchymal stem cells." in: European review for medical and pharmacological sciences, Vol. 20, Issue 24, pp. 5041-5048, (2017) (PubMed).

Wan, Wu, Zhang: "Local recurrence of small cell lung cancer following radiofrequency ablation is induced by HIF-1α expression in the transition zone." in: Oncology reports, Vol. 35, Issue 3, pp. 1297-308, (2016) (PubMed).

Ye, Peng, Hu, Yan, Chen, Xu: "In Vitro Differentiation of Bone Marrow Mesenchymal Stem Cells into Neuron-Like Cells by Cerebrospinal Fluid Improves Motor Function of Middle Cerebral Artery Occlusion Rats." in: Frontiers in neurology, Vol. 7, pp. 183, (2016) (PubMed).

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Liu, Cheng, Wang, Zhan, Xiong, Zhao: "Bone marrow-derived mesenchymal stem cells differentiate into nerve-like cells in vitro after transfection with brain-derived neurotrophic factor gene." in: In vitro cellular & developmental biology. Animal, Vol. 51, Issue 3, pp. 319-27, (2015) (PubMed).

Zhang, Zhou, Zhou, Xu, Liao, Yan, Lv, Luo: "Methyl 3,4-dihydroxybenzoate promotes neurite outgrowth of cortical neurons cultured in vitro." in: Neural regeneration research, Vol. 7, Issue 13, pp. 971-7, (2015) (PubMed).

Zhang, Chai, Liu, Xu, Yang: "Synergistic effects of Buyang Huanwu decoction and embryonic neural stem cell transplantation on the recovery of neurological function in a rat model of spinal cord injury." in: Experimental and therapeutic medicine, Vol. 9, Issue 4, pp. 1141-1148, (2015) (PubMed).

Wang, Li, Qiu, Jiang, Simon, Ma, Liu, Liu, Wang, Liang, Wu, Di, Zhou: "Anti-epileptic effect of Ganoderma lucidum polysaccharides by inhibition of intracellular calcium accumulation and stimulation of expression of CaMKII α in epileptic hippocampal neurons." in: PLoS ONE, Vol. 9, Issue 7, pp. e102161, (2015) (PubMed).

Wu, Chen, Guo, He, Hu: "Effects of transforming growth factor-?2 on myocilin expression and secretion in human primary cultured trabecular meshwork cells." in: International journal of clinical and experimental pathology, Vol. 7, Issue 8, pp. 4827-36, (2014) (PubMed).

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Huang, Jiang, Li, Deng, Li, Yao, Li, Zheng: "Using microfluidic chip to form brain-derived neurotrophic factor concentration gradient for studying neuron axon guidance." in: Biomicrofluidics, Vol. 8, Issue 1, pp. 14108, (2014) (PubMed).

Liu, Teng, Cui, Wang, Liu, Zhang: "Amyloid beta-derived diffusible ligands (ADDLs) induce abnormal expression of insulin receptors in rat hippocampal neurons." in: Journal of molecular neuroscience : MN, Vol. 52, Issue 1, pp. 124-30, (2014) (PubMed).

Cai, Zhang, Wang, Wang, Liu, Guo, Yang, Wang, Wang, Shu, Xiao: "BDNF attenuates IL-1?-induced F-actin remodeling by inhibiting NF-?B signaling in hippocampal neurons." in: Neuro endocrinology letters, Vol. 35, Issue 1, pp. 13-9, (2014) (PubMed).

Hu, Xiang, Wen, He, Wu, Hu: "Neuroprotective effect of Atractylodes macrocephalaon polysaccharides in vitro on neuronal apoptosis induced by hypoxia." in: Molecular medicine reports, Vol. 9, Issue 6, pp. 2573-81, (2014) (PubMed).

Zhao, Yu, Wu, Liu, Zhou, Gu, Zhang, Zhang, Wu: "Anticancer activity of SAHA, a potent histone deacetylase inhibitor, in NCI-H460 human large-cell lung carcinoma cells in vitro and in vivo." in: International journal of oncology, Vol. 44, Issue 2, pp. 451-8, (2014) (PubMed).

Xu, Sun, Wu, Xi: "Investigation of characteristics of feather follicle stem cells and their regeneration potential." in: Journal of stem cells & regenerative medicine, Vol. 7, Issue 2, pp. 69-74, (2014) (PubMed).

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Liu, Zhao, Wang, Zhang, Pei: "Optimal therapeutic dose and time window of picroside II in cerebral ischemic injury." in: Neural regeneration research, Vol. 9, Issue 15, pp. 1437-45, (2014) (PubMed).

Sun, Shao, Su, Zhao, Bi, Yang, Zhang, Gao, Miao: "Cholinergic neuron-like cells derived from bone marrow stromal cells induced by tricyclodecane-9-yl-xanthogenate promote functional recovery and neural protection after spinal cord injury." in: Cell transplantation, Vol. 22, Issue 6, pp. 961-75, (2013) (PubMed).

Details zu ENO2/NSE

Target: ENO2/NSE (ENO2) (Enolase 2 (Gamma, Neuronal) (ENO2))

Andere Bezeichnung: ENO2 (ENO2 Produkte)

Synonyme: ENO2 antikoerper, DKFZp459B1817 antikoerper, NSE antikoerper, AI837106 antikoerper, D6Ertd375e antikoerper, Eno-2 antikoerper, RNEN3 antikoerper, eno3 antikoerper, wu:fc09h05 antikoerper, zgc:92418 antikoerper, enolase 2 antikoerper, enolase 2 (gamma, neuronal) antikoerper, enolase 2, gamma neuronal antikoerper, enolase 2, gamma, neuronal antikoerper, ENO2 antikoerper, Eno2 antikoerper, eno2 antikoerper

Hintergrund: NSE(neuron specific enolase), also known as Enolase 2(ENO2), is found in elevated concentrations in plasma in certain neoplasias. The enolases catalyze the interconversion of 2-phosphoglycerate to phosphoenolpyruvate in the glycolytic pathway. ENO2 gene contains 12 exons distributed over 9,213 nucleotides. Human neurone-specific enolase is mapped to chromosome 12p13.

Synonyms: 2 phospho D glycerate hydrolyase antibody|2-phospho-D-glycerate hydro-lyase antibody|Eno 2 antibody|Eno2 antibody|ENOG antibody|ENOG_HUMAN antibody|Enolase 2 antibody|Enolase 2 gamma neuronal antibody|Enolase2 antibody|Gamma enolase antibody|Gamma-enolase antibody|Neural enolase antibody|Neuron specific enolase antibody|Neuron specific gamma enolase antibody|Neuron-specific enolase antibody|Neurone specific enolase antibody|NSE antibody

UniProt: P09104