NOX5 Antikörper (C-Term)

Produktnummer ABIN3042984

Produktdetails anti-NOX5 Antikörper

Target: NOX5 (NADPH Oxidase 5 (NOX5))

Bindungsspezifität: AA 747-765, C-Term

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser NOX5 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Verwendungszweck: Rabbit IgG polyclonal antibody for NADPH oxidase 5(NOX5) detection. Tested with WB, IHC-P, ICC in Human.

Sequenz: KVLKGHCEKF GFRFFQENF

Kreuzreaktivität (Details): No cross reactivity with other proteins.

Produktmerkmale: Rabbit IgG polyclonal antibody for NADPH oxidase 5(NOX5) detection. Tested with WB, IHC-P, ICC in Human.
Gene Name: NADPH oxidase, EF-hand calcium binding domain 5
Protein Name: NADPH oxidase 5

Aufreinigung: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human NOX5(747-765aa KVLKGHCEKFGFRFFQENF).

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
ICC: Concentration: 0.5-1 μg/mL, Tested Species: Human
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.

Kommentare:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P) and ICC.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Konservierungsmittel: Thimerosal (Merthiolate), Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Haltbarkeit: 12 months

Referenzen für anti-NOX5 Antikörper (ABIN3042984)

Jafari, Kim, Park, Li, Jang, Morris, Park, Huang: "CRISPR-Cas9 Mediated NOX4 Knockout Inhibits Cell Proliferation and Invasion in HeLa Cells." in: PLoS ONE, Vol. 12, Issue 1, pp. e0170327, (2017) (PubMed).

Details zu NOX5

Target: NOX5 (NADPH Oxidase 5 (NOX5))

Andere Bezeichnung: NOX5 (NOX5 Produkte)

Synonyme: NADPH oxidase 5 antikoerper, NOX5 antikoerper

Hintergrund: NOX5(Nadph Oxidase 5), also known as NOX5A or NOX5B, is a protein which in humans is encoded by the NOX5 gene. The International Radiation Hybrid Mapping Consortium mapped the NOX5 gene to chromosome 15. NOX5 is a novel NADPH oxidase that generates superoxide and functions as an H+ channel in a Ca2+-dependent manner. Banfi et al.(2001) found that, when heterologously expressed, NOX5 was quiescent in unstimulated cells. However, in response to elevations of the cytosolic Ca2+ concentration, it generated large amounts of superoxide. Using RT-PCR and Southern and Western blot analyses, Kamiguti et al.(2005) identified NOX5 as a flavin-containing Ca2+-dependent oxidase present in hairy leukemic cells(HC), but not normal B cells.

Synonyms: MGC149776 antibody|MGC149777 antibody|NADPH oxidase 5 antibody|NADPH oxidase EFhand calcium binding domain 5 antibody|NOX5 antibody|NOX5_HUMAN antibody

Pathways: Proton Transport