GLUT4 Antikörper (AA 491-509)

Produktnummer ABIN3032677

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch GLUT4 in WB und IHC (p). Er zeigt eine Reaktivität gegenüber Human, Maus und Ratte.

Kurzübersicht für GLUT4 Antikörper (AA 491-509) (ABIN3032677)

Target: GLUT4 (SLC2A4) (Solute Carrier Family 2 (Facilitated Glucose Transporter), Member 4 (SLC2A4))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser GLUT4 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-GLUT4 Antikörper

Bindungsspezifität: AA 491-509

Aufreinigung: Antigen affinity

Immunogen: Amino acids 491-509 (EQEVKPSTELEYLGPDEND) were used as the immunogen for this GLUT4 antibody (100% homologous in human, mouse and rat).

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: The stated application concentrations are suggested starting points. Titration of the GLUT4 antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 0.5-1 μg/mL,IHC (Paraffin): 0.5-1 μg/mL

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Buffer: 0.5 mg/mL if reconstituted with 0.2 mL sterile DI water

Lagerung: -20 °C

Informationen zur Lagerung: After reconstitution, the GLUT4 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Details zu GLUT4

Target: GLUT4 (SLC2A4) (Solute Carrier Family 2 (Facilitated Glucose Transporter), Member 4 (SLC2A4))

Andere Bezeichnung: GLUT4 (Glucose Transporter 4)

Hintergrund: Facilitated glucose transport by mammalian cells is not a property of a single protein but an activity associated with a family of structurally related proteins. Glucose transporter 4 is a insulin-responsive glucose transporter. It belongs to solute carrier family 2, member 1. Insulin alters the subcellular localization of GLUT4 vesicles in human muscle, and that this effect is impaired equally in insulin-resistant subjects with and without diabetes. A similar pattern of defects cause insulin resistance in human adipocytes. Human insulin resistance involves a defect in GLUT4 traffic and targeting leading to accumulation in a dense membrane compartment from which insulin is unable to recruit GLUT4 to the cell surface.

UniProt: P14672

Pathways: AMPK Signaling, Carbohydrate Homeostasis, Proton Transport, Brown Fat Cell Differentiation, Warburg Effekt