MAP3K3 Antikörper (N-Term)

Produktnummer ABIN3031851

Produktdetails anti-MAP3K3 Antikörper

Target: MAP3K3 (Mitogen-Activated Protein Kinase Kinase Kinase 3 (MAP3K3))

Bindungsspezifität: N-Term

Reaktivität: Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser MAP3K3 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Aufreinigung: Antigen affinity

Immunogen: An amino acid sequence from the N-terminus of mouse MAP3K3 (MKDLVALQMSRRTRL) was used as the immunogen for this MEKK3 antibody.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: The stated application concentrations are suggested starting amounts. Titration of the MEKK3 antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 0.5-1 μg/mL,IHC (Paraffin): 0.5-1 μg/mL

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Buffer: 0.5 mg/mL if reconstituted with 0.2 mL sterile DI water

Lagerung: -20 °C

Informationen zur Lagerung: After reconstitution, the MEKK3 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Details zu MAP3K3

Target: MAP3K3 (Mitogen-Activated Protein Kinase Kinase Kinase 3 (MAP3K3))

Andere Bezeichnung: MEKK3 (MAP3K3 Produkte)

Synonyme: MAPKKK3 antikoerper, MEKK3 antikoerper, AW548911 antikoerper, Mekk3 antikoerper, mKIAA4031 antikoerper, mitogen activated protein kinase kinase kinase 3 antikoerper, mitogen-activated protein kinase kinase kinase 3 antikoerper, Map3k3 antikoerper, MAP3K3 antikoerper, map3k3 antikoerper

Hintergrund: MAP3K3 (Mitogen-activated protein kinase kinase kinase 3), also known as MAP/ERK kinase kinase 3 (Mekk3), or MAPKKK3, is an enzyme that in humans is encoded by the MAP3K3 gene. MAP3K3 is contiguously distal to LYK5 on chromosome 17(Puffenberger et al., 2007). By yeast 2-hybrid analysis of a mouse T-cell cDNA library, Uhlik et al.(2003) showed that a C-terminal fragment of mouse Osm(CCM2) interacted with MAP3K3/Mekk3, which activates p38 in response to sorbitol-induced hyperosmotic conditions. Mekk3 and Osm colocalized in the cytoplasmic compartment of cotransfected cells, and the Mekk3-Osm complex was recruited to Rac1 and cytoskeletal actin-containing membrane ruffles in response to sorbitol treatment. Protein interaction assays showed that Osm interacted directly with the Mekk3 substrate Mkk3(MAP2K3), with actin, and with both GDP- and GTP-loaded Rac1. Uhlik et al.(2003) concluded that the RAC1-OSM-MEKK3-MKK3 complex is required for regulation of p38 activity in response to osmotic shock.

UniProt: Q61084

Pathways: MAPK Signalweg