The stated application concentrations are suggested starting amounts. Titration of the IKKb antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 0.5-1 μg/mL,IHC (Paraffin): 0.5-1 μg/mL
Beschränkungen
Nur für Forschungszwecke einsetzbar
Buffer
0.5 mg/mL if reconstituted with 0.2 mL sterile DI water
Lagerung
-20 °C
Informationen zur Lagerung
After reconstitution, the IKKb antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Target
IKBKB
(Inhibitor of kappa Light Polypeptide Gene Enhancer in B-Cells, Kinase beta (IKBKB))
I kappa B kinase beta, also known as IKKb or NFKBIKB, is a protein that in humans is encoded by the IKBKB gene.< a href=../search_result.php?search_txt=ikba>IkBabinds and inhibits NF-kB in the cytoplasm. Activated IKKb phosphorylates IkBa, which frees< a href=../search_result.php?search_txt=nfkb>NFkBto translocate to the nucleus. It is found that mutations in IKKb had a more pronounced effect upon NFKB activation than did comparable mutations in< a href=../search_result.php?search_txt=ikk%20alpha>IKKa.