HSPB2 Antikörper (Middle Region)

Produktnummer ABIN3031147

Produktdetails anti-HSPB2 Antikörper

Target: HSPB2 (Heat Shock 27kDa Protein 2 (HSPB2))

Bindungsspezifität: Middle Region

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser HSPB2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro))

Aufreinigung: Antigen affinity

Immunogen: An amino acid sequence from the middle region of human HSPB2 (RHPQRLDRHGFVSREFC) was used as the immunogen for this HSPB2 antibody (100% homologous in human, mouse and rat).

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: The stated application concentrations are suggested starting amounts. Titration of the HSPB2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 0.5-1 μg/mL,IHC (Frozen): 0.5-1 μg/mL,Immunocytochemistry: 0.5-1 μg/mL

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Buffer: 0.5 mg/mL if reconstituted with 0.2 mL sterile DI water

Lagerung: -20 °C

Informationen zur Lagerung: After reconstitution, the HSPB2 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Details zu HSPB2

Target: HSPB2 (Heat Shock 27kDa Protein 2 (HSPB2))

Andere Bezeichnung: HSPB2 (HSPB2 Produkte)

Synonyme: zgc:112532 antikoerper, HSP27 antikoerper, Hs.78846 antikoerper, LOH11CR1K antikoerper, MKBP antikoerper, 27kDa antikoerper, 2810021G24Rik antikoerper, heat shock protein family B (small) member 2 antikoerper, heat shock protein, alpha-crystallin-related, b2 antikoerper, heat shock protein 2 antikoerper, HSPB2 antikoerper, hspb2 antikoerper, Hspb2 antikoerper

Hintergrund: Alpha-B-crystallin is a member of the alpha-crystallin/small heat-shock protein (HSP) family and under various neuropathologic conditions accumulates in reactive astrocytes and degenerating neurons. In the 5-prime flanking region of the CRYAB gene on 11q22-q23, where a constitutive DNaseI hypersensitive site is located, Iwaki et al.(1997) identified a gene transcribed in the opposite direction. Analysis of its mRNA structure by RT-PCR and 5-prime/3-prime RACE revealed that this gene is composed of 2 exons and encodes a new member of the alpha-crystallin/hsp family. They called it Heat shock 27 protein 2 or Heat shock protein beta 2 and termed the gene HSPB2. Iwaki et al.(1997) also determined the complete genomic structure of the rat homolog of HSPB2. Southern blot analysis demonstrated that HSPB2 is expressed preferentially in skeletal muscle and heart but not in the lens, while the neighboring CRYABgene is highly expressed in all three tissues. The two related genes are arranged in a head-to-head manner with an intergenic sequence of less than 1 kb, raising a possibility of shared regulatory elements for their expression.

UniProt: Q16082