Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
Aufreinigung
Antigen affinity
Immunogen
An amino acid sequence from the C-terminus of human TNF Receptor-Associated Factor 4 (PKFISHQDIRKRNYVRDD) was used as the immunogen for this TRAF4 antibody (100% homologous in human, mouse and rat).
TRAF4
Reaktivität: Human, Maus
IF
Wirt: Kaninchen
Polyclonal
unconjugated
Applikationshinweise
The stated application concentrations are suggested starting amounts. Titration of the TRAF4 antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 0.5-1 μg/mL,IHC (Paraffin): 0.5-1 μg/mL
Beschränkungen
Nur für Forschungszwecke einsetzbar
Buffer
0.5 mg/mL if reconstituted with 0.2 mL sterile DI water
Lagerung
-20 °C
Informationen zur Lagerung
After reconstitution, the TRAF4 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
TNF Receptor-Associated Factor 4, also called CART1 or MLN62, is a protein that in humans is encoded by the TRAF4 gene. It is a member of the TNF receptor associated factor(TRAF) family, a family of scaffold proteins. Tomasetto et al.(1995) identified genes that are overexpressed in breast cancer by differential screening of cDNAs from breast cancer-derived metastatic axillary lymph nodes. The TRAF4 cDNA, which they designated MLN62, was mapped to 17q11-q12 by radioisotopic in situ hybridization. To investigate the biologic function of TRAF4, Regnier et al.(2002) generated Traf4-deficient mice by gene disruption.