PODXL Antikörper (Extracellular Domain)
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- Target Alle PODXL Antikörper anzeigen
- PODXL (Podocalyxin-Like (PODXL))
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Bindungsspezifität
- Extracellular Domain
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Reaktivität
- Human, Ratte, Kaninchen
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Wirt
- Maus
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Klonalität
- Monoklonal
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Konjugat
- Dieser PODXL Antikörper ist unkonjugiert
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Applikation
- Western Blotting (WB), Flow Cytometry (FACS), Immunoprecipitation (IP), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- Aufreinigung
- Protein G affinity chromatography
- Immunogen
- A human recombinant protein fragment containing the intracellular, transmembrane, and part of the extracellular domain was used as the immunogen for the Podocalyxin antibody.
- Klon
- 3D3
- Isotyp
- IgG1
- Top Product
- Discover our top product PODXL Primärantikörper
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- Applikationshinweise
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The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the Podocalyxin antibody to be titered up or down for optimal performance.
1. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. FACS: 0.5-1 μg/million cells,IF: 1-2 μg/mL,IP: 1-2 μg/500 μg protein,WB: 0.5-1 μg/mL,IHC (FFPE): 0.5-1 μg/mL for 30 min at RT (1),Prediluted format : incubate for 30 min at RT (2) - Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Konzentration
- 0.2 mg/mL
- Buffer
- 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide
- Konservierungsmittel
- Sodium azide
- Vorsichtsmaßnahmen
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Lagerung
- 4 °C,-20 °C
- Informationen zur Lagerung
- Store the Podocalyxin antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
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- Target
- PODXL (Podocalyxin-Like (PODXL))
- Andere Bezeichnung
- Podocalyxin (PODXL Produkte)
- Synonyme
- Gp200 antikoerper, PC antikoerper, PCLP antikoerper, PCLP-1 antikoerper, PCB antikoerper, AW121214 antikoerper, Ly102 antikoerper, Pclp1 antikoerper, podocalyxin antikoerper, MEP21 antikoerper, PODXL antikoerper, Gp135 antikoerper, PCLP1 antikoerper, cPCLP1 antikoerper, podxl antikoerper, fc18d02 antikoerper, wu:fc18d02 antikoerper, Pc antikoerper, Pcb antikoerper, Podxl antikoerper, podocalyxin like antikoerper, pyruvate carboxylase antikoerper, podocalyxin-like antikoerper, PODXL antikoerper, PC antikoerper, Podxl antikoerper, podxl antikoerper, Pcx antikoerper
- Hintergrund
- Podocalyxin is a member of the CD34 transmembrane sialomucin family. It is over-expressed on the podocyte foot projections and plays essential roles in kidney development and homeostasis, blood filtration and urine formation. It is also expressed on vascular endothelia, hematopoietic progenitors and a subset of neurons. Overexpression may be linked to more aggressive tumor behavior. Podocalyxin antibody can identify podocytes in the urine (podocyturia) that may indicate glomerular disease, pre-eclampsia, and other kidney pathology.
- Gen-ID
- 5420
- Pathways
- Tube Formation
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