Cytochrome C Antikörper (AA 1-80)
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- Target Alle Cytochrome C (CYCS) Antikörper anzeigen
- Cytochrome C (CYCS) (Cytochrome C, Somatic (CYCS))
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Bindungsspezifität
- AA 1-80
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Reaktivität
- Human, Ratte, Maus, Pferd, Hund, Taube, Drosophila melanogaster, Frosch
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Wirt
- Maus
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Klonalität
- Monoklonal
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Konjugat
- Dieser Cytochrome C Antikörper ist unkonjugiert
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Applikation
- Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- Produktmerkmale
- It recognizes an epitope within amino acids 93-104 of pigeon Cytochrome C, a well-characterized mobile electron transport protein that is essential to energy conversion in all aerobic organisms. In mammalian cells, this highly conserved protein is normally localized to the mitochondrial inter-membrane space. More recent studies have identified cytosolic cytochrome c as a factor necessary for activation of apoptosis. During apoptosis, cytochrome c is trans-located from the mitochondrial membrane to the cytosol, where it is required for activation of caspase-3 (CPP32). Overexpression of Bcl-2 has been shown to prevent the translocation of cytochrome c, thereby blocking the apoptotic process. Overexpression of Bax has been shown to induce the release of cytochrome c and to induce cell death. The release of cytochrome c from the mitochondria is thought to trigger an apoptotic cascade, whereby Apaf-1 binds to Apaf-3 (caspase-9) in a cytochrome c-dependent manner, leading to caspase-9 cleavage of caspase-3. This mAb recognizes total cytochrome C which includes both apocytochrome (i.e. cytochrome in the cytosol without heme attached) and holocytochrome (i.e cytochrome in the mitochondria with heme attached).
- Aufreinigung
- Protein G affinity chromatography
- Immunogen
- Synthetic peptides corresponding to amino acid 1-80, 81-104 and 66-104 of pigeon Cytochrome C was used as the immunogen for the Cytochrome C antibody.
- Klon
- 7H8-2C12
- Isotyp
- IgG2b kappa
- Top Product
- Discover our top product CYCS Primärantikörper
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- Applikationshinweise
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Optimal dilution of the Cytochrome C antibody should be determined by the researcher.
1. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 0.5-1 μg/mL,Western blot: 0.5-1 μg/mL,Immunohistochemistry (FFPE): 0.25-0.5 μg/mL for 30 min at RT (1),Prediluted format: incubate for 30 min at RT (2) - Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Konzentration
- 0.2 mg/mL
- Buffer
- 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide
- Konservierungsmittel
- Sodium azide
- Vorsichtsmaßnahmen
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Lagerung
- 4 °C,-20 °C
- Informationen zur Lagerung
- Store the Cytochrome C antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
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- Target
- Cytochrome C (CYCS) (Cytochrome C, Somatic (CYCS))
- Andere Bezeichnung
- Cytochrome C (CYCS Produkte)
- Synonyme
- CYC antikoerper, HCS antikoerper, THC4 antikoerper, cyct antikoerper, cyc antikoerper, hcs antikoerper, CYCS antikoerper, cytc antikoerper, CYCSA antikoerper, CG17903 antikoerper, Cyt C antikoerper, Cyt c antikoerper, Cyt-c2 antikoerper, CytC-2 antikoerper, Cytc antikoerper, Cytc-p antikoerper, DC4 antikoerper, DMc01 antikoerper, Dmel\\CG17903 antikoerper, cyt c antikoerper, cyt c-p antikoerper, cyt-c-p antikoerper, cyt-c2 antikoerper, cyt.c antikoerper, cytochrome c antikoerper, dc4 antikoerper, pDMc01 antikoerper, wu:fk52b01 antikoerper, zgc:86706 antikoerper, ENSMUSG00000058927 antikoerper, cytochrome c, somatic antikoerper, cytochrome c, somatic S homeolog antikoerper, Cytochrome c proximal antikoerper, cytochrome c, somatic b antikoerper, cytochrome c, somatic pseudogene antikoerper, cytochrome c-like antikoerper, cytochrome c antikoerper, CYCS antikoerper, cycs.S antikoerper, cycs antikoerper, Cycs antikoerper, Cyt-c-p antikoerper, cycsb antikoerper, Gm10053 antikoerper, LOC100724240 antikoerper, CYC antikoerper, LOC100847700 antikoerper, LOC101107954 antikoerper, LOC106829744 antikoerper, LOC106992390 antikoerper, LOC102085712 antikoerper
- Hintergrund
- It recognizes an epitope within amino acids 93-104 of pigeon Cytochrome C, a well-characterized mobile electron transport protein that is essential to energy conversion in all aerobic organisms. In mammalian cells, this highly conserved protein is normally localized to the mitochondrial inter-membrane space. More recent studies have identified cytosolic cytochrome c as a factor necessary for activation of apoptosis. During apoptosis, cytochrome c is trans-located from the mitochondrial membrane to the cytosol, where it is required for activation of caspase-3 (CPP32). Overexpression of Bcl-2 has been shown to prevent the translocation of cytochrome c, thereby blocking the apoptotic process. Overexpression of Bax has been shown to induce the release of cytochrome c and to induce cell death. The release of cytochrome c from the mitochondria is thought to trigger an apoptotic cascade, whereby Apaf-1 binds to Apaf-3 (caspase-9) in a cytochrome c-dependent manner, leading to caspase-9 cleavage of caspase-3. This mAb recognizes total cytochrome C which includes both apocytochrome (i.e. cytochrome in the cytosol without heme attached) and holocytochrome (i.e cytochrome in the mitochondria with heme attached).
- Pathways
- Apoptose, Caspase Kaskade in der Apoptose, Positive Regulation of Endopeptidase Activity
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