Histone Antikörper

Produktnummer ABIN3024805

Produktdetails

Target: Histone

Reaktivität: Human, Ratte, Maus

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Flow Cytometry (FACS), Immunofluorescence (IF)

Aufreinigung: Protein G affinity chromatography

Immunogen: Nuclei of human leukemia biopsy cells were used as the immunogen for the Histone antibody.

Klon: AE-4

Isotyp: IgG2a kappa

Anwendungsinformationen

Applikationshinweise: Optimal dilution of the Histone antibody should be determined by the researcher.

1. Staining of formalin/paraffin tissues requires boiling tissue sections in 10  mM Citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 min.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Western blot: 0.5-1 μg/mL, Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 0.5-1 μg/mL,Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT (1),Prediluted format : incubate for 30 min at RT (2)

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.2 mg/mL

Buffer: 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store the Histone antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Antigendetails

Target: Histone

Synonyme: Histone H2A antikoerper, Histone H2B 1 antikoerper, Histone H3 antikoerper, Probable histone H2B 4 antikoerper, Histone H2B 2 antikoerper, Histone antikoerper, his-16 antikoerper, his-12 antikoerper, his-11 antikoerper, his-9 antikoerper, his-43 antikoerper, his-61 antikoerper, his-48 antikoerper, his-57 antikoerper, his-22 antikoerper, his-4 antikoerper, his-3 antikoerper, his-42 antikoerper, his-66 antikoerper, his-65 antikoerper, Histone antikoerper

Hintergrund: Eukaryotic histones are basic and water-soluble nuclear proteins that form hetero-octameric nucleosome particles by wrapping 146 base pairs of DNA in a left-handed super-helical turn sequentially to form chromosomal fiber. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form the octamer, formed of two H2A-H2B dimers and two H3-H4 dimers, forming two nearly symmetrical halves by tertiary structure. Over 80 % of nucleosomes contain the linker Histone H1, derived from an intronless gene that interacts with linker DNA between nucleosomes and mediates compaction into higher order chromatin. Histones are subject to posttranslational modification by enzymes primarily on their N-terminal tails, but also in their globular domains. Such modifications include methylation, citrullination, acetylation, phosphorylation, sumoylation, ubiquitination and ADP-ribosylation.