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Actin Antikörper

ACTA1 Reaktivität: Human, Ratte WB, IHC (p), IF Wirt: Maus Monoclonal PMAC1-1 unconjugated
Produktnummer ABIN3024129
  • Target Alle Actin (ACTA1) Antikörper anzeigen
    Actin (ACTA1) (Actin, alpha 1, Skeletal Muscle (ACTA1))
    Reaktivität
    • 96
    • 44
    • 43
    • 17
    • 12
    • 10
    • 9
    • 8
    • 6
    • 6
    • 5
    • 5
    • 4
    • 3
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Human, Ratte
    Wirt
    • 81
    • 31
    Maus
    Klonalität
    • 76
    • 34
    Monoklonal
    Konjugat
    • 63
    • 12
    • 12
    • 10
    • 5
    • 4
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Dieser Actin Antikörper ist unkonjugiert
    Applikation
    • 91
    • 57
    • 40
    • 28
    • 27
    • 25
    • 10
    • 9
    • 7
    • 2
    • 1
    • 1
    Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF)
    Aufreinigung
    Protein G affinity chromatography
    Immunogen
    An amino acid sequence common among human muscle actins was used as the immunogen for the Muscle Actin antibody.
    Klon
    PMAC1-1
    Isotyp
    IgG1 kappa
    Top Product
    Discover our top product ACTA1 Primärantikörper
  • Applikationshinweise
    Titering of the Muscle Actin antibody may be required for optimal performance.

    1. FFPE testing requires sections to be boiled in  pH 6 10  mM citrate buffer for 10-20 minutes, followed by cooling at RT for 20 minutes, prior to staining.
    2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Western blot: 0.5-1 μg/mL,Immunofluorescence: 1-2 μg/mL,Immunohistochemistry (FFPE): 1-2 μg/mL for 30 min at RT (1),Prediluted format: incubate for 30 min at RT (2)

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Konzentration
    0.2 mg/mL
    Buffer
    0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide
    Konservierungsmittel
    Sodium azide
    Vorsichtsmaßnahmen
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Lagerung
    4 °C,-20 °C
    Informationen zur Lagerung
    Store the Muscle Actin antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
  • Target
    Actin (ACTA1) (Actin, alpha 1, Skeletal Muscle (ACTA1))
    Andere Bezeichnung
    Muscle Actin (ACTA1 Produkte)
    Synonyme
    ACTA antikoerper, ASMA antikoerper, CFTD antikoerper, CFTD1 antikoerper, CFTDM antikoerper, MPFD antikoerper, NEM1 antikoerper, NEM2 antikoerper, NEM3 antikoerper, acta antikoerper, asma antikoerper, cftd antikoerper, cftd1 antikoerper, cftdm antikoerper, mpfd antikoerper, nem1 antikoerper, AA959943 antikoerper, Acta-2 antikoerper, Acts antikoerper, Actsk-1 antikoerper, actc antikoerper, actc1 antikoerper, cfk antikoerper, hm:zeh0631 antikoerper, zeh0631 antikoerper, acta1 antikoerper, hm:zeh0293 antikoerper, zgc:112098 antikoerper, actin, alpha 1, skeletal muscle antikoerper, actin, alpha 1, skeletal muscle L homeolog antikoerper, actin, alpha 1b, skeletal muscle antikoerper, actin, muscle antikoerper, actin, alpha skeletal muscle antikoerper, actin, alpha 1a, skeletal muscle antikoerper, ACTA1 antikoerper, acta1.L antikoerper, acta1 antikoerper, Acta1 antikoerper, acta1b antikoerper, LOC100533357 antikoerper, LOC109479779 antikoerper, LOC109108280 antikoerper, acta1a antikoerper
    Hintergrund
    Actin participates in many important cellular processes, including muscle contraction, cell motility, cell division and cytokinesis, vesicle and organelle movement, cell signaling, and the establishment and maintenance of cell junctions and cell shape. An actin protein's mass is roughly 42-  kDa and it is the monomeric subunit of two types of filaments in cells: microfilaments, one of the three major components of the cytoskeleton, and thin filaments, part of the contractile apparatus in muscle cells. It can be present as either a free monomer called G-actin (globular) or as part of a linear polymer microfilament called F-actin (filamentous), both of which are essential for such important cellular functions as the mobility and contraction of cells during cell division. [Wiki]

    This antibody recognizes actin of skeletal, cardiac, and smooth muscle cells.

    Pathways
    Caspase Kaskade in der Apoptose, Myometrial Relaxation and Contraction, Skeletal Muscle Fiber Development
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