Maus anti-Human IgG (Fc Region), (Heavy Chain) Antikörper

Details zu Produkt Nr. ABIN302085, Anbieter: Anmelden zum Anzeigen
Antigen
Epitop
Fc Region, Heavy Chain
6302
1956
1804
1065
888
727
375
301
35
34
31
15
11
7
3
3
1
1
1
1
1
1
1
Reaktivität
Human
3072
2887
2377
1634
1481
785
690
459
456
397
369
364
301
288
280
146
128
114
42
34
29
26
16
15
11
10
8
7
5
5
4
4
3
3
3
3
3
3
3
2
2
2
1
1
1
1
1
1
1
Wirt
Maus
6987
5101
1927
1047
535
361
90
84
55
35
23
15
8
5
3
1
1
Klonalität (Klon)
Monoklonal ()
Applikation
Immunocytochemistry (ICC), ELISA, Western Blotting (WB)
Optionen
Hersteller
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Hersteller Produkt- Nr.
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Immunogen Fusion protein of human IgG Fc fragment.
Klon EM-07
Isotyp IgG1
Spezifität The antibody EM-07 reacts with Fc part of human IgG heavy chain and with isolated Fc fragments.
Kreuzreaktivität (Details) The antibody EM-07 does not crossreact with IgM.
Reinigung Purified from hybridoma culture supernatant by protein-A affinity chromatography.
Reinheit > 95 % (by SDS-PAGE)
Antigen
Hintergrund Immunoglobulin G (IgG) is a 150 kDa soluble protein that serves as a major effector molecule of the humoral immune response in man. Its concentration in blood plasma of healthy individuals is approximately 10 g/l, which accounts for about 75 % of the total plasma immunoglobulins. IgG has the highest stability of blood immunoglobulins (T1/2 = 21 days) and is able of placental transfer. IgG is secreted by plasma cells at a comparably high rate as other immunoglobulins.
Molekulargewicht 150 kDa
Applikations-hinweise Western Blotting:
Application note: The peroxidase conjugate of this antibody is suitable for detection of human IgG Fc fragments by WB.
ELISA:
Application note: The peroxidase conjugate of this antibody is suitable for detection of human IgG Fc fragments by ELISA.

Working concentrations should be determined by the investigator.
Beschränkungen Nur für Forschungszwecke einsetzbar
Format Liquid
Konzentration 1 mg/mL
Buffer Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
Konservierungs-mittel Sodium azide
Vorsichtsmaßnahmen This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handhabung Do not freeze.
Lagerung 4 °C
Informationen zur Lagerung Store at 2-8 °C. Do not use after expiration date stamped on vial label.
Bilder des Herstellers
Western Blotting (WB) image for Mouse anti-Human IgG (Fc Region), (Heavy Chain) antibody (ABIN302085) IgG κ light chain (1), IgG λ light chain (2) and IgG Fc fragment (3) purifi...
Allgemeine Veröffentlichungen Brinkmann, Heusser: "T cell-dependent differentiation of human B cells into IgM, IgG, IgA, or IgE plasma cells: high rate of antibody production by IgE plasma cells, but limited clonal expansion of IgE precursors." in: Cellular immunology, Vol. 152, Issue 2, pp. 323-32, 1994 (PubMed).

Balogh, Merétey, Falus, Bozsóky: "Serological abnormalities in juvenile chronic arthritis: a review of 46 cases." in: Annals of the rheumatic diseases, Vol. 39, Issue 2, pp. 129-34, 1980 (PubMed).

Franklin: "Structure and function of immunoglobulins." in: Acta endocrinologica. Supplementum, Vol. 194, pp. 77-95, 1975 (PubMed).

Fuller, Keyser: "Serum immunoglobulins after surgical operation." in: Clinical chemistry, Vol. 21, Issue 6, pp. 667-71, 1975 (PubMed).