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HLA-E Antikörper

HLA-E Reaktivität: Human FACS, IP Wirt: Maus Monoclonal MEM-E-08 unconjugated
Produktnummer ABIN302071
  • Target Alle HLA-E Antikörper anzeigen
    HLA-E (HLA Class I Histocompatibility Antigen, alpha Chain E (HLA-E))
    Reaktivität
    • 71
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    Human
    Wirt
    • 44
    • 28
    Maus
    Klonalität
    • 46
    • 26
    Monoklonal
    Konjugat
    • 31
    • 6
    • 4
    • 4
    • 4
    • 4
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Dieser HLA-E Antikörper ist unkonjugiert
    Applikation
    • 43
    • 29
    • 20
    • 13
    • 13
    • 12
    • 10
    • 9
    • 3
    • 2
    • 2
    • 1
    • 1
    Flow Cytometry (FACS), Immunoprecipitation (IP)
    Spezifität
    The antibody MEM-E/08 recognizes an extracellular epitope on native surface-expressed HLA-E, but not denaturated heavy chain of HLA-E. HLA-E belongs to the MHC Class I molecules (MHC Class Ib, nonclassical) and it is expressed on many types of the human cells. The published results revealed that antibody is remarkably specific for HLA-E, only with weak cross-reactivity with following classical MHC Class I molecules (MHC Class Ia): HLA-A24, -B7, -B27, -B51, -B54, -C7.
    Kreuzreaktivität (Details)
    Human
    Aufreinigung
    Purified by protein-A affinity chromatography.
    Reinheit
    > 95 % (by SDS-PAGE)
    Immunogen
    Bacterially expressed recombinant HLA-E refolded with beta2-microglobulin and peptide.
    Klon
    MEM-E-08
    Isotyp
    IgG1
    Top Product
    Discover our top product HLA-E Primärantikörper
  • Applikationshinweise
    Flow cytometry: Recommended dilution: 4 μg/mL, positive control: human cell line LCL 721.221 (HLA-E transfected).
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Konzentration
    1 mg/mL
    Buffer
    Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
    Konservierungsmittel
    Sodium azide
    Vorsichtsmaßnahmen
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handhabung
    Do not freeze.
    Lagerung
    4 °C
    Informationen zur Lagerung
    Store at 2-8°C. Do not freeze.
  • Nachmani, Zimmermann, Oiknine Djian, Weisblum, Livneh, Khanh Le, Galun, Horejsi, Isakov, Shomron, Wolf, Hengel, Mandelboim: "MicroRNA editing facilitates immune elimination of HCMV infected cells." in: PLoS pathogens, Vol. 10, Issue 2, pp. e1003963, (2014) (PubMed).

    Allard, Oger, Vignard, Percier, Fregni, Périer, Caignard, Charreau, Bernardeau, Khammari, Dréno, Gervois: "Serum soluble HLA-E in melanoma: a new potential immune-related marker in cancer." in: PLoS ONE, Vol. 6, Issue 6, pp. e21118, (2011) (PubMed).

    Lo Monaco, Tremante, Cerboni, Melucci, Sibilio, Zingoni, Nicotra, Natali, Giacomini: "Human leukocyte antigen E contributes to protect tumor cells from lysis by natural killer cells." in: Neoplasia (New York, N.Y.), Vol. 13, Issue 9, pp. 822-30, (2011) (PubMed).

    Gonen-Gross, Achdout, Arnon, Gazit, Stern, Horejsí, Goldman-Wohl, Yagel, Mandelboim: "The CD85J/leukocyte inhibitory receptor-1 distinguishes between conformed and beta 2-microglobulin-free HLA-G molecules." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 175, Issue 8, pp. 4866-74, (2005) (PubMed).

    Palmisano, Contardi, Morabito, Gargaglione, Ferrara, Pistillo: "HLA-E surface expression is independent of the availability of HLA class I signal sequence-derived peptides in human tumor cell lines." in: Human immunology, Vol. 66, Issue 1, pp. 1-12, (2004) (PubMed).

  • Target
    HLA-E (HLA Class I Histocompatibility Antigen, alpha Chain E (HLA-E))
    Andere Bezeichnung
    HLA-E (HLA-E Produkte)
    Synonyme
    EA1.2 antikoerper, EA2.1 antikoerper, HLA-6.2 antikoerper, MHC antikoerper, QA1 antikoerper, major histocompatibility complex, class I, E antikoerper, HLA-E antikoerper, PATR-E antikoerper
    Hintergrund
    Major histocompatibility complex, class I, E,HLA-E (human leukocyte antigen E) is a non-classical MHC I antigen, which is important for dialogue with NK cells and their regulation through interaction with CD94/NKG2 receptor. Like other MHC I molecules, transmembrane HLA-E molecule (45 kDa) associates with beta2 microglobulin. Unlike HLA-G, expression of HLA-E molecules is not so restricted, but it has been detected at least at mRNA level in virtually all cells and tissues examined. In peripheral blood, HLA-E protein is expressed at least in all mononuclear cells, but in different quantity (B cells and monocytes more than T cells and NK cells).,HLA class I histocompatibility antigen, alpha chain E, MHC class I antigen E
    Gen-ID
    3133
    UniProt
    P13747
    Pathways
    Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process
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