WNT2B Antikörper

Produktnummer ABIN293249

Produktdetails anti-WNT2B Antikörper

Target: WNT2B (Wingless-Type MMTV Integration Site Family, Member 2B (WNT2B))

Reaktivität: Human, Maus

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser WNT2B Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunocytochemistry (ICC)

Spezifität: Selected for its ability to recognize mouse and human Wnt-2b in the applications listed.

Aufreinigung: Affinity purified

Immunogen: E. coli-derived rmWnt-2b.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Approved: ELISA, ICC, WB

Usage: Western Blot: This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Wnt-2b. The detection limit for rmWnt-2b is approximately 5 ng/lane and 1 ng/lane under non-reducing and reducing conditions, respectively. In this format, this antibody shows approximately 20 % cross-reactivity with rhWnt-2, and less than 5 % cross-reactivity with rhWnt-7a, -9a, rmWnt-1, -3a, -4, -5a, -5b, -8a, -8b, -9b, -10a, -10b, -11, -16. Immunocytochemistry: This antibody has been used at a concentration of 10 μg/mL to detect Wnt-2b in BT20 human breast carcinoma cells. Cells were fixed with PBS containing 4 % paraformaldehyde for 20 minutes at room temperature and blocked with PBS containing 10 % normal donkey serum, 0.1 % Triton X-100, and 1 % BSA for 45 minutes at room temperature. After blocking, cells were incubated with diluted primary antibody overnight at 4? C followed by NorthernLights-557-coupled anti-goat IgG at room temperature in the dark for one hour. Between each step, cells were washed with PBS containing BSA. Direct ELISA: This antibody can be used at 0.5-1.0 μg/mL with the appropriate secondary reagents to detect mouse Wnt-2b. The detection limit for rmWnt-2b is approximately 0.5 ng/well.

Kommentare:

Target Species of Antibody: Mouse

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: deionized water

Konzentration: Lot specific

Buffer: Lyophilized.

Details zu WNT2B

Target: WNT2B (Wingless-Type MMTV Integration Site Family, Member 2B (WNT2B))

Andere Bezeichnung: WNT2B (WNT2B Produkte)

Synonyme: WNT13 antikoerper, XWNT2 antikoerper, wnt2b antikoerper, XWnt-2 antikoerper, Xwnt-2b antikoerper, wnt-2b antikoerper, xwnt2b antikoerper, WNT2B antikoerper, Wnt13 antikoerper, Wnt family member 2B antikoerper, wingless-type MMTV integration site family, member 2Ba antikoerper, Wnt family member 2B L homeolog antikoerper, wingless-type MMTV integration site family, member 2B antikoerper, wingless-type MMTV integration site family, member 2Bb antikoerper, WNT2B antikoerper, Wnt2b antikoerper, wnt2ba antikoerper, wnt2b.L antikoerper, wnt2bb antikoerper

Hintergrund: Name/Gene ID: WNT2B
Family: WNT

Synonyms: WNT2B, Protein Wnt-2b, XWNT2, Protein Wnt-13, WNT13, XWNT2, Xenopus, homolog of

Gen-ID: 7482

UniProt: Q93097

Pathways: WNT Signalweg